Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10327704" target="_blank" >RIV/00216208:11310/16:10327704 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027006:_____/16:00003665

Výsledek na webu

<a href="http://dx.doi.org/10.3389/fmich.2015.01046" target="_blank" >http://dx.doi.org/10.3389/fmich.2015.01046</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmich.2015.01046" target="_blank" >10.3389/fmich.2015.01046</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities

Popis výsledku v původním jazyce

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T putrescentiae and one mixed population of T putrescentiae and T fanetzhangorum collected from different habitats. Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes. Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardiniurn (five populations), Bartonella-like (five populations), Blattabacteriurn-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacteriurn. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia infested populations. Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea like in the eggs of T putrescentiae indicates mother to offspring (vertical) transmission. Results of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T putrescentiae.

Název v anglickém jazyce

Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities

Popis výsledku anglicky

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T putrescentiae and one mixed population of T putrescentiae and T fanetzhangorum collected from different habitats. Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes. Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardiniurn (five populations), Bartonella-like (five populations), Blattabacteriurn-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacteriurn. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia infested populations. Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea like in the eggs of T putrescentiae indicates mother to offspring (vertical) transmission. Results of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T putrescentiae.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

—

Projekt

<a href="/cs/project/GA15-09038S" target="_blank" >GA15-09038S: Jsou asociované bakterie s roztočem Tyrophagus putrescentiae odpovědné za úspěšnou kolonizaci prostředí domácností?</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

JUL

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

19

Strana od-do

—

Kód UT WoS článku

000379465900001

EID výsledku v databázi Scopus

2-s2.0-84983084906