Ethylenediaminetetraacetic Acid, Sodium Citrate, Heparin and Citrate Phosphate Dextrose-Adenine Anticoagulants Differentially Affect Cytokine mRNA Expression in Blood Leukocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10330202" target="_blank" >RIV/00216208:11310/16:10330202 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.7754/Clin.Lab.2016.151140" target="_blank" >http://dx.doi.org/10.7754/Clin.Lab.2016.151140</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.7754/Clin.Lab.2016.151140" target="_blank" >10.7754/Clin.Lab.2016.151140</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ethylenediaminetetraacetic Acid, Sodium Citrate, Heparin and Citrate Phosphate Dextrose-Adenine Anticoagulants Differentially Affect Cytokine mRNA Expression in Blood Leukocytes

Popis výsledku v původním jazyce

Background: The aim of this study is to determine whether and how the anticoagulant agents EDTA, sodium citrate, and heparin present in vacutainers and CPDA located in transfusion bags modify peripheral blood cell behavior. Methods: We compared the effect of four anticoagulants within an expression study quantifying mRNA by realtime PCR in 60 blood samples. Results: We observed a significant increase of TNF alpha and CCL2 mRNA expression in leukocytes placed in EDTA anticoagulant compared to leukocytes collected with citrate or heparin (p <= 0.5). Compared to blood cells influenced by one of the three anticoagulant agents present in vacuum tubes, cells from transfusion bags affected by CPDA displayed significantly lower levels of CCL2 and MYC mRNA, whereas levels of IL-10 and TNF alpha mRNA were much higher (p <= 0.5). Conclusions: The anticoagulant EDTA significantly increases proinflammatory cytokine mRNA in freshly isolated PBMC. Anticoagulant CPDA significantly alters levels of some of the tested mRNAs in comparison to blood cells drawn into one of the three anticoagulant agents present in vacutainers.

Název v anglickém jazyce

Ethylenediaminetetraacetic Acid, Sodium Citrate, Heparin and Citrate Phosphate Dextrose-Adenine Anticoagulants Differentially Affect Cytokine mRNA Expression in Blood Leukocytes

Popis výsledku anglicky

Background: The aim of this study is to determine whether and how the anticoagulant agents EDTA, sodium citrate, and heparin present in vacutainers and CPDA located in transfusion bags modify peripheral blood cell behavior. Methods: We compared the effect of four anticoagulants within an expression study quantifying mRNA by realtime PCR in 60 blood samples. Results: We observed a significant increase of TNF alpha and CCL2 mRNA expression in leukocytes placed in EDTA anticoagulant compared to leukocytes collected with citrate or heparin (p <= 0.5). Compared to blood cells influenced by one of the three anticoagulant agents present in vacuum tubes, cells from transfusion bags affected by CPDA displayed significantly lower levels of CCL2 and MYC mRNA, whereas levels of IL-10 and TNF alpha mRNA were much higher (p <= 0.5). Conclusions: The anticoagulant EDTA significantly increases proinflammatory cytokine mRNA in freshly isolated PBMC. Anticoagulant CPDA significantly alters levels of some of the tested mRNAs in comparison to blood cells drawn into one of the three anticoagulant agents present in vacutainers.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinical Laboratory

ISSN

1433-6510

e-ISSN

—

Svazek periodika

62

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

4

Strana od-do

1371-1374

Kód UT WoS článku

000385813300026

EID výsledku v databázi Scopus

2-s2.0-84981187663