Development of a Ratiometric Fluorescent Probe with Two Reactive Sulfoxides for Monitoring the Activity of Methionine Sulfoxide Reductase A

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10374847" target="_blank" >RIV/00216208:11310/18:10374847 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1055/s-0036-1591888" target="_blank" >https://doi.org/10.1055/s-0036-1591888</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1055/s-0036-1591888" target="_blank" >10.1055/s-0036-1591888</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of a Ratiometric Fluorescent Probe with Two Reactive Sulfoxides for Monitoring the Activity of Methionine Sulfoxide Reductase A

Popis výsledku v původním jazyce

Biological oxidation of methionine side chains in proteins is a process that affects the functions of many proteins. One of the key regulators of this signaling is the enzyme methionine sulfoxide reductase A (MsrA). MsrA is implicated in a number of diseases, but detailed understanding of its function is hindered by the lack of tools for monitoring the enzyme&apos;s activity. We have designed and synthesized a probe named (S, S)-Sulfox-2 that is based on a BODIPY fluorophore and is equipped with two chiral sulfoxide units of defined stereochemistry. (S, S)-Sulfox-2 is shown to be highly responsive to MsrA and allows tracing of the MsrA activity by a significant change in the fluorescence profile.

Název v anglickém jazyce

Development of a Ratiometric Fluorescent Probe with Two Reactive Sulfoxides for Monitoring the Activity of Methionine Sulfoxide Reductase A

Popis výsledku anglicky

Biological oxidation of methionine side chains in proteins is a process that affects the functions of many proteins. One of the key regulators of this signaling is the enzyme methionine sulfoxide reductase A (MsrA). MsrA is implicated in a number of diseases, but detailed understanding of its function is hindered by the lack of tools for monitoring the enzyme&apos;s activity. We have designed and synthesized a probe named (S, S)-Sulfox-2 that is based on a BODIPY fluorophore and is equipped with two chiral sulfoxide units of defined stereochemistry. (S, S)-Sulfox-2 is shown to be highly responsive to MsrA and allows tracing of the MsrA activity by a significant change in the fluorescence profile.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

<a href="/cs/project/GJ17-25897Y" target="_blank" >GJ17-25897Y: Vývoj fluorescenčních substrátů pro methionin sulfoxid reduktázu a zobecnění designu pro další enzymy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Synthesis

ISSN

0039-7881

e-ISSN

—

Svazek periodika

50

Číslo periodika v rámci svazku

04

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

772-777

Kód UT WoS článku

000424152700009

EID výsledku v databázi Scopus

2-s2.0-85040793408