Amperometric Determination of Catecholamines by Enzymatic Biosensors in Flow Systems
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10376275" target="_blank" >RIV/00216208:11310/18:10376275 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61388955:_____/18:00490697
Výsledek na webu
<a href="https://doi.org/10.1002/elan.201800078" target="_blank" >https://doi.org/10.1002/elan.201800078</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elan.201800078" target="_blank" >10.1002/elan.201800078</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Amperometric Determination of Catecholamines by Enzymatic Biosensors in Flow Systems
Popis výsledku v původním jazyce
Four various biosensors based on flow enzymatic mini-reactors containing one of the two different mesoporous silica powders (SBA-15 or MCM-41) modified by one of the two enzymes (laccase or tyrosinase) were used for the determination of L-DOPA, dopamine, noradrenaline, and adrenaline. Dependences of peak currents on detection potentials, flow rates, injected volumes, reactor volumes, and analyte concentrations were studied and optimized. The silica powder of MCM-41 with covalently bonded laccase (Lac) was found to be the best filling for the detection of catecholamines. Relative current response of such biosensor was: 100% for dopamine, 32.1% for L-DOPA, 26.2% for noradrenaline, and 0.71% for adrenaline. Statistical evaluation of parallel determinations of studied analytes with this biosensor proved its good accuracy (RSD=1.82-5.18%) and sensitivity (5.46-15.5molL(-1)). Life time of the studied biosensors is several months. The practical applicability of Lac-MCM41 biosensor was successfully verified by the determination of dopamine and noradrenaline in medical solutions for infusions.
Název v anglickém jazyce
Amperometric Determination of Catecholamines by Enzymatic Biosensors in Flow Systems
Popis výsledku anglicky
Four various biosensors based on flow enzymatic mini-reactors containing one of the two different mesoporous silica powders (SBA-15 or MCM-41) modified by one of the two enzymes (laccase or tyrosinase) were used for the determination of L-DOPA, dopamine, noradrenaline, and adrenaline. Dependences of peak currents on detection potentials, flow rates, injected volumes, reactor volumes, and analyte concentrations were studied and optimized. The silica powder of MCM-41 with covalently bonded laccase (Lac) was found to be the best filling for the detection of catecholamines. Relative current response of such biosensor was: 100% for dopamine, 32.1% for L-DOPA, 26.2% for noradrenaline, and 0.71% for adrenaline. Statistical evaluation of parallel determinations of studied analytes with this biosensor proved its good accuracy (RSD=1.82-5.18%) and sensitivity (5.46-15.5molL(-1)). Life time of the studied biosensors is several months. The practical applicability of Lac-MCM41 biosensor was successfully verified by the determination of dopamine and noradrenaline in medical solutions for infusions.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Electroanalysis
ISSN
1040-0397
e-ISSN
—
Svazek periodika
30
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
9
Strana od-do
1163-1171
Kód UT WoS článku
000435271000026
EID výsledku v databázi Scopus
2-s2.0-85043703846