Anti-adhesive properties of nanocrystalline diamond films against Escherichia coli bacterium: Influence of surface termination and cultivation medium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10385879" target="_blank" >RIV/00216208:11310/18:10385879 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/18:00498303

Výsledek na webu

<a href="https://doi.org/10.1016/j.diamond.2018.02.001" target="_blank" >https://doi.org/10.1016/j.diamond.2018.02.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.diamond.2018.02.001" target="_blank" >10.1016/j.diamond.2018.02.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Anti-adhesive properties of nanocrystalline diamond films against Escherichia coli bacterium: Influence of surface termination and cultivation medium

Popis výsledku v původním jazyce

Recent interest in industrial and medical usage of carbon nanomaterials arises the need for approved testing protocols for characterizing their interaction with living systems. Nanocrystalline diamond (NCD) films were chosen as a promising material for such investigation due to their unique mechanical properties, chemical stability and biocompatibility. In this study, we examined the anti-adhesive properties of differently terminated NCD films deposited by plasma-enhanced CVD on glass or silicon against Escherichia colt bacterium. We focused on the influence of cultivation medium in this process and compared bacterial adhesion to H-, O- and F-terminated NCD films in complex Luria-Bertani (LB) and mineral (M9) cultivation media that substantially differ in the content of organic molecules and thus in the potential of conditioning film formation. We found the properties of NCD films to be independent of the base substrate, as the amount of biofilm was comparable for both NCD films deposited on glass and on silicon. The anti-adhesive effect was observed only in the mineral medium where hydrogen and fluorine terminated NCD films reduced bacterial adhesion by ca. 50%. In complex medium we did not observe the reduction of bacterial adhesion. These differences are attributed to the passivation of the H- or F-terminated NCD films by organic molecules adsorbed from the complex medium. For O-terminated NCD films, no anti-adhesive effect was observed, regardless of the cultivation medium. Our results show that the growth of Escherichia colt in mineral cultivation medium can be affected by the diamond atomic termination.

Název v anglickém jazyce

Anti-adhesive properties of nanocrystalline diamond films against Escherichia coli bacterium: Influence of surface termination and cultivation medium

Popis výsledku anglicky

Recent interest in industrial and medical usage of carbon nanomaterials arises the need for approved testing protocols for characterizing their interaction with living systems. Nanocrystalline diamond (NCD) films were chosen as a promising material for such investigation due to their unique mechanical properties, chemical stability and biocompatibility. In this study, we examined the anti-adhesive properties of differently terminated NCD films deposited by plasma-enhanced CVD on glass or silicon against Escherichia colt bacterium. We focused on the influence of cultivation medium in this process and compared bacterial adhesion to H-, O- and F-terminated NCD films in complex Luria-Bertani (LB) and mineral (M9) cultivation media that substantially differ in the content of organic molecules and thus in the potential of conditioning film formation. We found the properties of NCD films to be independent of the base substrate, as the amount of biofilm was comparable for both NCD films deposited on glass and on silicon. The anti-adhesive effect was observed only in the mineral medium where hydrogen and fluorine terminated NCD films reduced bacterial adhesion by ca. 50%. In complex medium we did not observe the reduction of bacterial adhesion. These differences are attributed to the passivation of the H- or F-terminated NCD films by organic molecules adsorbed from the complex medium. For O-terminated NCD films, no anti-adhesive effect was observed, regardless of the cultivation medium. Our results show that the growth of Escherichia colt in mineral cultivation medium can be affected by the diamond atomic termination.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Diamond and Related Materials

ISSN

0925-9635

e-ISSN

—

Svazek periodika

83

Číslo periodika v rámci svazku

March 2018

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

87-93

Kód UT WoS článku

000430767200013

EID výsledku v databázi Scopus

2-s2.0-85041464379