Changes in the activity of some metabolic enzymes in the heart of SHR rat incurred by transgenic expression of CD36

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10387200" target="_blank" >RIV/00216208:11310/18:10387200 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/18:00493613

Výsledek na webu

<a href="https://doi.org/10.1007/s13105-018-0641-1" target="_blank" >https://doi.org/10.1007/s13105-018-0641-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13105-018-0641-1" target="_blank" >10.1007/s13105-018-0641-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Changes in the activity of some metabolic enzymes in the heart of SHR rat incurred by transgenic expression of CD36

Popis výsledku v původním jazyce

Hypertension, dyslipidemia, and insulin resistance in the spontaneously hypertensive rat (SHR) can be alleviated by rescuing CD36 fatty acid translocase. The present study investigated whether transgenic rescue of CD36 in SHR could affect mitochondrial function and activity of selected metabolic enzymes in the heart. These analyses were conducted on ventricular preparations derived from SHR and from transgenic strain SHR-Cd36 that expresses a functional wild-type CD36. Our respirometric measurements revealed that mitochondria isolated from the left ventricles exhibited two times higher respiratory activity than those isolated from the right ventricles. Whereas, we did not observe any significant changes in functioning of the mitochondrial respiratory system between both rat strains, enzyme activities of total hexokinase, and both mitochondrial and total malate dehydrogenase were markedly decreased in the left ventricles of transgenic rats, compared to SHR. We also detected downregulated expression of the succinate dehydrogenase subunit SdhB (complex II) and 70 kDa peroxisomal membrane protein in the left ventricles of SHR-Cd36. These data indicate that CD36 may affect in a unique fashion metabolic substrate flexibility of the left and right ventricles.

Název v anglickém jazyce

Changes in the activity of some metabolic enzymes in the heart of SHR rat incurred by transgenic expression of CD36

Popis výsledku anglicky

Hypertension, dyslipidemia, and insulin resistance in the spontaneously hypertensive rat (SHR) can be alleviated by rescuing CD36 fatty acid translocase. The present study investigated whether transgenic rescue of CD36 in SHR could affect mitochondrial function and activity of selected metabolic enzymes in the heart. These analyses were conducted on ventricular preparations derived from SHR and from transgenic strain SHR-Cd36 that expresses a functional wild-type CD36. Our respirometric measurements revealed that mitochondria isolated from the left ventricles exhibited two times higher respiratory activity than those isolated from the right ventricles. Whereas, we did not observe any significant changes in functioning of the mitochondrial respiratory system between both rat strains, enzyme activities of total hexokinase, and both mitochondrial and total malate dehydrogenase were markedly decreased in the left ventricles of transgenic rats, compared to SHR. We also detected downregulated expression of the succinate dehydrogenase subunit SdhB (complex II) and 70 kDa peroxisomal membrane protein in the left ventricles of SHR-Cd36. These data indicate that CD36 may affect in a unique fashion metabolic substrate flexibility of the left and right ventricles.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30105 - Physiology (including cytology)

Projekt

<a href="/cs/project/GB14-36804G" target="_blank" >GB14-36804G: Centrum mitochondriální biologie a patologie (MITOCENTRUM)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Physiology and Biochemistry

ISSN

1138-7548

e-ISSN

—

Svazek periodika

74

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

ES - Španělské království

Počet stran výsledku

11

Strana od-do

479-489

Kód UT WoS článku

000440827500011

EID výsledku v databázi Scopus

2-s2.0-85048690725