Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10393467" target="_blank" >RIV/00216208:11310/19:10393467 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=6w1YL-Ck1P" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=6w1YL-Ck1P</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pntd.0007078" target="_blank" >10.1371/journal.pntd.0007078</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva

Popis výsledku v původním jazyce

Background Hosts repeatedly bitten by sand flies develop antibodies against sand fly saliva and screening of these immunoglobulins can be employed to estimate the risk of Leishmania transmission, to indicate the feeding preferences of sand flies, or to evaluate the effectiveness of vector control campaigns. Previously, antibodies to sand fly saliva were detected using whole salivary gland homogenate (SGH) or recombinant proteins, both of which also have their disadvantages. This is the first study on sand flies where short peptides designed based on salivary antigens were successfully utilized for antibody screening. Methodology/Principal findings Specific IgG was studied in hosts naturally exposed to Phlebotomus orientalis, the main vector of Leishmania donovani in East Africa. Four peptides were designed by the commercial program EpiQuest-B, based on the sequences of the two most promising salivary antigens, yellow-related protein and ParSP25-like protein. Short amino acid peptides were synthesised and modified for ELISA experiments. Specific anti-P. orientalis IgG was detected in sera of dogs, goats, and sheep from Ethiopia. The peptide OR24 P2 was shown to be suitable for antibody screening; it correlated positively with SGH and its specificity and sensitivity were comparable or even better than that of previously published recombinant proteins. Conclusions/Significance OR24 P2, the peptide based on salivary antigen of P. orientalis, was shown to be a valuable tool for antibody screening of domestic animals naturally exposed to P. orientalis. We suggest the application of this promising methodology using species-specific short peptides to other sand fly-host combinations. Author summary Previously, two types of antigens were used for detection of antibodies to sand flies: 1) salivary gland homogenate (SGH), which requires maintaining a sand fly colony and laborious work to obtain a sufficient amount of antigen or 2) recombinant proteins with the need to use cell expression and a complicated purification procedure. In contrast, synthetic peptides have never been studied for sand flies despite it being easier to produce them in sufficient quantities and purity. In this study, we screened specific antibodies to sand flies in domestic animals using synthetic peptides based on the two most antigenic salivary proteins of Phlebotomus orientalis. This sand fly is the main East African vector of Leishmania donovani, causative agent of visceral leishmaniasis, and we detected specific anti-P. orientalis IgG in naturally exposed dogs, goats, and sheep from Ethiopia. We showed that, in dogs and goats, the peptide named OR24 P2 is more suitable for antibody detection then the recombinant proteins. Therefore, we recommend this peptide to replace SGH in larger epidemiological studies for evaluation of the effectiveness of vector control programmes or to estimate the risk of Leishmania transmission.

Název v anglickém jazyce

Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva

Popis výsledku anglicky

Background Hosts repeatedly bitten by sand flies develop antibodies against sand fly saliva and screening of these immunoglobulins can be employed to estimate the risk of Leishmania transmission, to indicate the feeding preferences of sand flies, or to evaluate the effectiveness of vector control campaigns. Previously, antibodies to sand fly saliva were detected using whole salivary gland homogenate (SGH) or recombinant proteins, both of which also have their disadvantages. This is the first study on sand flies where short peptides designed based on salivary antigens were successfully utilized for antibody screening. Methodology/Principal findings Specific IgG was studied in hosts naturally exposed to Phlebotomus orientalis, the main vector of Leishmania donovani in East Africa. Four peptides were designed by the commercial program EpiQuest-B, based on the sequences of the two most promising salivary antigens, yellow-related protein and ParSP25-like protein. Short amino acid peptides were synthesised and modified for ELISA experiments. Specific anti-P. orientalis IgG was detected in sera of dogs, goats, and sheep from Ethiopia. The peptide OR24 P2 was shown to be suitable for antibody screening; it correlated positively with SGH and its specificity and sensitivity were comparable or even better than that of previously published recombinant proteins. Conclusions/Significance OR24 P2, the peptide based on salivary antigen of P. orientalis, was shown to be a valuable tool for antibody screening of domestic animals naturally exposed to P. orientalis. We suggest the application of this promising methodology using species-specific short peptides to other sand fly-host combinations. Author summary Previously, two types of antigens were used for detection of antibodies to sand flies: 1) salivary gland homogenate (SGH), which requires maintaining a sand fly colony and laborious work to obtain a sufficient amount of antigen or 2) recombinant proteins with the need to use cell expression and a complicated purification procedure. In contrast, synthetic peptides have never been studied for sand flies despite it being easier to produce them in sufficient quantities and purity. In this study, we screened specific antibodies to sand flies in domestic animals using synthetic peptides based on the two most antigenic salivary proteins of Phlebotomus orientalis. This sand fly is the main East African vector of Leishmania donovani, causative agent of visceral leishmaniasis, and we detected specific anti-P. orientalis IgG in naturally exposed dogs, goats, and sheep from Ethiopia. We showed that, in dogs and goats, the peptide named OR24 P2 is more suitable for antibody detection then the recombinant proteins. Therefore, we recommend this peptide to replace SGH in larger epidemiological studies for evaluation of the effectiveness of vector control programmes or to estimate the risk of Leishmania transmission.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

<a href="/cs/project/GA17-10308S" target="_blank" >GA17-10308S: Slinné proteiny flebotoma Sergentomyia schwetzi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS Neglected Tropical Diseases [online]

ISSN

1935-2735

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

e0007078

Kód UT WoS článku

000457398700042

EID výsledku v databázi Scopus

2-s2.0-85060549968