Electrochemical determination of indole-3-acetic acid and indole-3-butyric acid using HPLC with carbon felt detector

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10395082" target="_blank" >RIV/00216208:11310/19:10395082 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=bmV_hkzO-k" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=bmV_hkzO-k</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-019-2375-3" target="_blank" >10.1007/s00706-019-2375-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical determination of indole-3-acetic acid and indole-3-butyric acid using HPLC with carbon felt detector

Popis výsledku v původním jazyce

Method for the determination of two auxins, indole-3-acetic acid and indole-3-butyric acid, is developed using HPLC with electrochemical detection. As the working electrode, recently developed carbon felt detector with high conversion degree was employed. Optimum conditions, i.e. separation on 125-4 Lichrospher 100, RP-18 column with mobile phase consisting of buffer pH 4.0 and methanol (40:60, v/v) and detection at potential +1.5V, allow to obtain linear concentration dependences in the concentration range from 1 to 100 mu moldm(-3), with determination limits of 1.1 mu moldm(-3) and 1.8 mu moldm(-3) for indole-3-acetic and -butyric acid, respectively. Applicability of the method was verified by the determination of both analytes in rooting preparation; the results were in agreement with the reference method, although not with the values declared by the manufacturer.

Název v anglickém jazyce

Electrochemical determination of indole-3-acetic acid and indole-3-butyric acid using HPLC with carbon felt detector

Popis výsledku anglicky

Method for the determination of two auxins, indole-3-acetic acid and indole-3-butyric acid, is developed using HPLC with electrochemical detection. As the working electrode, recently developed carbon felt detector with high conversion degree was employed. Optimum conditions, i.e. separation on 125-4 Lichrospher 100, RP-18 column with mobile phase consisting of buffer pH 4.0 and methanol (40:60, v/v) and detection at potential +1.5V, allow to obtain linear concentration dependences in the concentration range from 1 to 100 mu moldm(-3), with determination limits of 1.1 mu moldm(-3) and 1.8 mu moldm(-3) for indole-3-acetic and -butyric acid, respectively. Applicability of the method was verified by the determination of both analytes in rooting preparation; the results were in agreement with the reference method, although not with the values declared by the manufacturer.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA17-03868S" target="_blank" >GA17-03868S: Nové metody elektrochemického sledování biologicky aktivních organických látek v environmentálních, biologických a potravinových matricích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

—

Svazek periodika

150

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

4

Strana od-do

439-442

Kód UT WoS článku

000461394400010

EID výsledku v databázi Scopus

2-s2.0-85061098092