Vše

Co hledáte?

Vše
Projekty
Výsledky výzkumu
Subjekty

Rychlé hledání

  • Projekty podpořené TA ČR
  • Významné projekty
  • Projekty s nejvyšší státní podporou
  • Aktuálně běžící projekty

Chytré vyhledávání

  • Takto najdu konkrétní +slovo
  • Takto z výsledků -slovo zcela vynechám
  • “Takto můžu najít celou frázi”

Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10403319" target="_blank" >RIV/00216208:11310/19:10403319 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=uePbcynBjF" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=uePbcynBjF</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1371/journal.pntd.0007832" target="_blank" >10.1371/journal.pntd.0007832</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis

  • Popis výsledku v původním jazyce

    Author summary Canine leishmaniasis (CanL) is a widespread severe chronic disease in dogs, caused by the protozoan parasite Leishmania infantum. Leishmania parasites are transmitted by the bite of phlebotomine sand flies, of which the most important one in southwestern Europe is Phlebotomus perniciosus. A sand fly bite is accompanied by the inoculation of saliva, which elicits a specific antibody response in the host. Past studies have used these antibodies as markers of exposure to sand flies. Recently, a rapid sand fly exposure test was prepared using one salivary protein of P. perniciosus, the SP03B protein. This rapid test possessed a high sensitivity and specificity when screening experimentally exposed versus non-bitten dogs. However, since naturally exposed dogs possess a higher variability of antibodies against sand fly saliva, we have optimized the previously proposed rapid test and evaluated its detectability on field samples. In this study we present the optimized rSP03B sero-strip that possesses a higher specificity and an ability to detect seropositive dogs from a naturally exposed dog population in a similar fashion as standard serological methods. Furthermore, we have optimized it with whole canine blood which renders it suitable for use in field conditions. Background Canine leishmaniasis (CanL) is a severe chronic disease caused by Leishmania infantum and transmitted by sand flies of which the main vector in the Western part of the Mediterranean basin is Phlebotomus perniciosus. Previously, an immunochromatographic test (ICT) was proposed to allow rapid evaluation of dog exposure to P. perniciosus. In the present study, we optimized the prototype and evaluated the detection accuracy of the ICT in field conditions. Possible cross-reactions with other hematophagous arthropods were also assessed. Methodology/Principal findings The ICT was optimized by expressing the rSP03B protein in a HEK293 cell line, which delivered an increased specificity (94.92%). The ICT showed an excellent reproducibility and inter-person reliability, and was optimized for use with whole canine blood which rendered an excellent degree of agreement with the use of serum. Field detectability of the ICT was assessed by screening 186 dogs from different CanL endemic areas with both the SGH-ELISA and the ICT, and 154 longitudinally sampled dogs only with the ICT. The ICT results corresponded to the SGH-ELISA for most areas, depending on the statistical measure used. Furthermore, the ICT was able to show a clear seasonal fluctuation in the proportion of bitten dogs. Finally, we excluded cross-reactions between non-vector species and confirmed favorable cross-reactions with other L. infantum vectors belonging to the subgenus Larroussius.

  • Název v anglickém jazyce

    Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis

  • Popis výsledku anglicky

    Author summary Canine leishmaniasis (CanL) is a widespread severe chronic disease in dogs, caused by the protozoan parasite Leishmania infantum. Leishmania parasites are transmitted by the bite of phlebotomine sand flies, of which the most important one in southwestern Europe is Phlebotomus perniciosus. A sand fly bite is accompanied by the inoculation of saliva, which elicits a specific antibody response in the host. Past studies have used these antibodies as markers of exposure to sand flies. Recently, a rapid sand fly exposure test was prepared using one salivary protein of P. perniciosus, the SP03B protein. This rapid test possessed a high sensitivity and specificity when screening experimentally exposed versus non-bitten dogs. However, since naturally exposed dogs possess a higher variability of antibodies against sand fly saliva, we have optimized the previously proposed rapid test and evaluated its detectability on field samples. In this study we present the optimized rSP03B sero-strip that possesses a higher specificity and an ability to detect seropositive dogs from a naturally exposed dog population in a similar fashion as standard serological methods. Furthermore, we have optimized it with whole canine blood which renders it suitable for use in field conditions. Background Canine leishmaniasis (CanL) is a severe chronic disease caused by Leishmania infantum and transmitted by sand flies of which the main vector in the Western part of the Mediterranean basin is Phlebotomus perniciosus. Previously, an immunochromatographic test (ICT) was proposed to allow rapid evaluation of dog exposure to P. perniciosus. In the present study, we optimized the prototype and evaluated the detection accuracy of the ICT in field conditions. Possible cross-reactions with other hematophagous arthropods were also assessed. Methodology/Principal findings The ICT was optimized by expressing the rSP03B protein in a HEK293 cell line, which delivered an increased specificity (94.92%). The ICT showed an excellent reproducibility and inter-person reliability, and was optimized for use with whole canine blood which rendered an excellent degree of agreement with the use of serum. Field detectability of the ICT was assessed by screening 186 dogs from different CanL endemic areas with both the SGH-ELISA and the ICT, and 154 longitudinally sampled dogs only with the ICT. The ICT results corresponded to the SGH-ELISA for most areas, depending on the statistical measure used. Furthermore, the ICT was able to show a clear seasonal fluctuation in the proportion of bitten dogs. Finally, we excluded cross-reactions between non-vector species and confirmed favorable cross-reactions with other L. infantum vectors belonging to the subgenus Larroussius.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10600 - Biological sciences

Návaznosti výsledku

  • Projekt

  • Návaznosti

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2019

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    PLoS Neglected Tropical Diseases [online]

  • ISSN

    1935-2735

  • e-ISSN

  • Svazek periodika

    13

  • Číslo periodika v rámci svazku

    11

  • Stát vydavatele periodika

    US - Spojené státy americké

  • Počet stran výsledku

    16

  • Strana od-do

    e0007832

  • Kód UT WoS článku

    000503278100048

  • EID výsledku v databázi Scopus

    2-s2.0-85076196981