Evaluation of separation properties of stationary phases in supercritical fluid chromatography; deazapurine nucleosides case study
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10403675" target="_blank" >RIV/00216208:11310/19:10403675 - isvavai.cz</a>
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=MH4CKxocfk" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=MH4CKxocfk</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.microc.2019.104137" target="_blank" >10.1016/j.microc.2019.104137</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Evaluation of separation properties of stationary phases in supercritical fluid chromatography; deazapurine nucleosides case study
Popis výsledku v původním jazyce
A series of biologically important 7-deazapurine nucleosides was used for evaluation of selectivity of three stationary phases designed for supercritical fluid chromatography. The sorbents of stationary phases tested were diethylamine, diol and 2-picolylamine bonded on ethylene bridged hybrid particles. The highest selectivity for the tested nucleosides showed neutral diol column. All twelve tested nucleosides were baseline resolved in simple mobile phase composed of carbon dioxide/ethanol 79/21 (v/v). The effect of basic isopropylamine and mixed isopropylamine and trifluoroacetic acid mobile phase additives on retention and selectivity was also assessed. The interactions participating in the separation systems were revealed by linear free energy relationship. The obtained results demonstrated the significant contribution of hydrogen bond acidity and basicity on chromatographic behavior of the diol- and 2-picolylamine-based stationary phases.
Název v anglickém jazyce
Evaluation of separation properties of stationary phases in supercritical fluid chromatography; deazapurine nucleosides case study
Popis výsledku anglicky
A series of biologically important 7-deazapurine nucleosides was used for evaluation of selectivity of three stationary phases designed for supercritical fluid chromatography. The sorbents of stationary phases tested were diethylamine, diol and 2-picolylamine bonded on ethylene bridged hybrid particles. The highest selectivity for the tested nucleosides showed neutral diol column. All twelve tested nucleosides were baseline resolved in simple mobile phase composed of carbon dioxide/ethanol 79/21 (v/v). The effect of basic isopropylamine and mixed isopropylamine and trifluoroacetic acid mobile phase additives on retention and selectivity was also assessed. The interactions participating in the separation systems were revealed by linear free energy relationship. The obtained results demonstrated the significant contribution of hydrogen bond acidity and basicity on chromatographic behavior of the diol- and 2-picolylamine-based stationary phases.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Microchemical Journal
ISSN
0026-265X
e-ISSN
—
Svazek periodika
150
Číslo periodika v rámci svazku
November
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
6
Strana od-do
104137
Kód UT WoS článku
000495469400060
EID výsledku v databázi Scopus
2-s2.0-85069898772