Online preconcentration of weak electrolytes at the pH boundary induced by a system zone in capillary zone electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10404620" target="_blank" >RIV/00216208:11310/19:10404620 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=pSsSZe9csv" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=pSsSZe9csv</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2019.08.004" target="_blank" >10.1016/j.aca.2019.08.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Online preconcentration of weak electrolytes at the pH boundary induced by a system zone in capillary zone electrophoresis

Popis výsledku v původním jazyce

Within the frame of the dynamic pH junction preconcentration technique in capillary electrophoresis, we introduce a novel approach based on the use of the pH boundary of a system zone for the preconcentration of general, multivalent, weak analytes in a system of binary, uni-univalent, background electrolytes (BGE). For such purpose, in addition to presenting a comprehensive flowchart for the development of a method for BGE preconcentration, we showed several model cases using acidic, basic and ampholytic analytes. Furthermore, we combined the flowchart with calculations in electrophoretic software PeakMaster to determine all necessary information such as analyte mobility, system zones and the amplitude of the pH boundary of a system zone as a function of the sample matrix. For an even more detailed understanding of the process, we also investigated changes in the pH boundary through computer simulations with Simul 5, providing an in-depth characterization of all model analytes according to the steps of the flowchart and to PeakMaster calculations for experimental verification of the final BGE preconcentration.

Název v anglickém jazyce

Online preconcentration of weak electrolytes at the pH boundary induced by a system zone in capillary zone electrophoresis

Popis výsledku anglicky

Within the frame of the dynamic pH junction preconcentration technique in capillary electrophoresis, we introduce a novel approach based on the use of the pH boundary of a system zone for the preconcentration of general, multivalent, weak analytes in a system of binary, uni-univalent, background electrolytes (BGE). For such purpose, in addition to presenting a comprehensive flowchart for the development of a method for BGE preconcentration, we showed several model cases using acidic, basic and ampholytic analytes. Furthermore, we combined the flowchart with calculations in electrophoretic software PeakMaster to determine all necessary information such as analyte mobility, system zones and the amplitude of the pH boundary of a system zone as a function of the sample matrix. For an even more detailed understanding of the process, we also investigated changes in the pH boundary through computer simulations with Simul 5, providing an in-depth characterization of all model analytes according to the steps of the flowchart and to PeakMaster calculations for experimental verification of the final BGE preconcentration.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA16-05942S" target="_blank" >GA16-05942S: Gradientová chromatofokusace a izoelektrická fokusace - nástroje pro separaci proteinů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

—

Svazek periodika

1085

Číslo periodika v rámci svazku

November

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

126-135

Kód UT WoS článku

000485779200014

EID výsledku v databázi Scopus

2-s2.0-85070279797