Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10409344" target="_blank" >RIV/00216208:11310/19:10409344 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/19:00112932 RIV/67985904:_____/19:00537765

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=BLdyGWgf_h" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=BLdyGWgf_h</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/biolre/ioz117" target="_blank" >10.1093/biolre/ioz117</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes

Popis výsledku v původním jazyce

Culture media used in assisted reproduction are commonly supplemented with gonadotropin hormones to support the nuclear and cytoplasmic maturation of in vitro matured oocytes. However, the effect of gonadotropins on protein synthesis in oocytes is yet to be fully understood. As published data have previously documented a positive in vitro effect of follicle-stimulating hormone (FSH) on cytoplasmic maturation, we exposed mouse denuded oocytes to FSH in order to evaluate the changes in global protein synthesis. We found that dose-dependent administration of FSH resulted in a decrease of methionine incorporation into de novo synthesized proteins in denuded mouse oocytes and oocytes cultured in cumulus-oocyte complexes. Similarly, FSH influenced methionine incorporation in additional mammalian species including human. Furthermore, we showed the expression of FSH-receptor protein in oocytes. We found that major translational regulators were not affected by FSH treatment; however, the amino acid uptake became impaired. We propose that the effect of FSH treatment on amino acid uptake is influenced by FSH receptor with the effect on oocyte metabolism and physiology. Summary Sentence FSH treatment decrease methionine incorporation into de novo synthesized proteins in mouse, porcine, and bovine oocytes, and FSHR protein is expressed in oocytes and 2cell embryo.

Název v anglickém jazyce

Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes

Popis výsledku anglicky

Culture media used in assisted reproduction are commonly supplemented with gonadotropin hormones to support the nuclear and cytoplasmic maturation of in vitro matured oocytes. However, the effect of gonadotropins on protein synthesis in oocytes is yet to be fully understood. As published data have previously documented a positive in vitro effect of follicle-stimulating hormone (FSH) on cytoplasmic maturation, we exposed mouse denuded oocytes to FSH in order to evaluate the changes in global protein synthesis. We found that dose-dependent administration of FSH resulted in a decrease of methionine incorporation into de novo synthesized proteins in denuded mouse oocytes and oocytes cultured in cumulus-oocyte complexes. Similarly, FSH influenced methionine incorporation in additional mammalian species including human. Furthermore, we showed the expression of FSH-receptor protein in oocytes. We found that major translational regulators were not affected by FSH treatment; however, the amino acid uptake became impaired. We propose that the effect of FSH treatment on amino acid uptake is influenced by FSH receptor with the effect on oocyte metabolism and physiology. Summary Sentence FSH treatment decrease methionine incorporation into de novo synthesized proteins in mouse, porcine, and bovine oocytes, and FSHR protein is expressed in oocytes and 2cell embryo.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology of Reproduction

ISSN

0006-3363

e-ISSN

—

Svazek periodika

101

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

719-732

Kód UT WoS článku

000509503400008

EID výsledku v databázi Scopus

2-s2.0-85075813083