Flow amperometric biosensor based on two enzymatic reactors (acetylcholinesterasecholine oxidase) for the detection of neurotransmitter acetylcholine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10409590" target="_blank" >RIV/00216208:11310/19:10409590 - isvavai.cz</a>

Výsledek na webu

<a href="http://web.natur.cuni.cz/analchem/isc/isc15.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc15.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Flow amperometric biosensor based on two enzymatic reactors (acetylcholinesterasecholine oxidase) for the detection of neurotransmitter acetylcholine

Popis výsledku v původním jazyce

A stable amperometric biosensor with separated bienzymatic (acetylcholinesterase (AChE)-choline oxidase (ChOx)) part has been successfully developed for the determination of acetylcholine (ACh) in FIA for the first time. The bienzymatic part consists of two consecutively connected enzymatic reactors, which were independently prepared by covalent immobilization of AChE or ChOx on mesoporous silica powder (SBA-15). The amperometric measurements are based on the detection of consumed oxygen. The wall-jet cell with working silver solid amalgam electrode covered by mercury film to carry out four-electron oxygen reduction at the highly negative potential was used. The experimental parameters such as amounts of immobilized enzymes, pH of carrier solution, detection potential, flow rate, and injection volume of ACh were optimized. The detection limit was found to be 4.1 μmol L-1. The developed amperometric ACh biosensor showed good repeatability and long-term stability. The same enzymatic reactors were successfully used for 500 measurements during 90 days.

Název v anglickém jazyce

Flow amperometric biosensor based on two enzymatic reactors (acetylcholinesterasecholine oxidase) for the detection of neurotransmitter acetylcholine

Popis výsledku anglicky

A stable amperometric biosensor with separated bienzymatic (acetylcholinesterase (AChE)-choline oxidase (ChOx)) part has been successfully developed for the determination of acetylcholine (ACh) in FIA for the first time. The bienzymatic part consists of two consecutively connected enzymatic reactors, which were independently prepared by covalent immobilization of AChE or ChOx on mesoporous silica powder (SBA-15). The amperometric measurements are based on the detection of consumed oxygen. The wall-jet cell with working silver solid amalgam electrode covered by mercury film to carry out four-electron oxygen reduction at the highly negative potential was used. The experimental parameters such as amounts of immobilized enzymes, pH of carrier solution, detection potential, flow rate, and injection volume of ACh were optimized. The detection limit was found to be 4.1 μmol L-1. The developed amperometric ACh biosensor showed good repeatability and long-term stability. The same enzymatic reactors were successfully used for 500 measurements during 90 days.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 15th International Students Conference &quot;Modern Analytical Chemistry&quot;

ISBN

978-80-7444-068-7

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

61-66

Název nakladatele

Univerzita Karlova, Přírodovědecká fakulta

Místo vydání

Praha

Místo konání akce

Prague

Datum konání akce

19. 9. 2019

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000576787700010