High-diversity microbiomes in the guts of bryophagous beetles (Coleoptera: Byrrhidae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10413316" target="_blank" >RIV/00216208:11310/19:10413316 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/19:00520531 RIV/61988987:17310/19:A2002363

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ojUP5fBboK" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ojUP5fBboK</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14411/eje.2019.044" target="_blank" >10.14411/eje.2019.044</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High-diversity microbiomes in the guts of bryophagous beetles (Coleoptera: Byrrhidae)

Popis výsledku v původním jazyce

The diversity and role of the gut microbiota of insects is a rapidly growing field of entomology, primarily fueled by new metagenomic techniques. Whereas endosymbionts in the guts of xylophagous or herbivorous insects are well studied, the microbiomes in moss-eating (bryophagous) insects remain uncharacterized. Using the Illumina MiSeq platform, we determined the composition of microbiomes in the gut, abdomen and on the body surface of two bryophagous species: Simplocaria semistriata (Fabricius, 1794) and Curimopsis paleata (Erichson, 1846) (Coleoptera: Byrrhidae). Gut microbiomes differed substantially from abdominal microbiomes in the same individuals, which indicates the need to separate them during dissection. Microbiomes in the gut and abdomen differed markedly from surface microbial assemblages. Gut microbiomes in bryophages had the highest MOTU richness, diversity and relative rarity. The eudominant bacteria in the guts and abdomens of bryophages were Novosphingobium, Bradyrhizobium, Ralstonia and Caulobacter, which are responsible for the detoxification of secondary metabolites or nitrogen fixation. These are less common in the surface samples and, therefore, likely to be associated with the specific ability of bryophages to feed on mosses.

Název v anglickém jazyce

High-diversity microbiomes in the guts of bryophagous beetles (Coleoptera: Byrrhidae)

Popis výsledku anglicky

The diversity and role of the gut microbiota of insects is a rapidly growing field of entomology, primarily fueled by new metagenomic techniques. Whereas endosymbionts in the guts of xylophagous or herbivorous insects are well studied, the microbiomes in moss-eating (bryophagous) insects remain uncharacterized. Using the Illumina MiSeq platform, we determined the composition of microbiomes in the gut, abdomen and on the body surface of two bryophagous species: Simplocaria semistriata (Fabricius, 1794) and Curimopsis paleata (Erichson, 1846) (Coleoptera: Byrrhidae). Gut microbiomes differed substantially from abdominal microbiomes in the same individuals, which indicates the need to separate them during dissection. Microbiomes in the gut and abdomen differed markedly from surface microbial assemblages. Gut microbiomes in bryophages had the highest MOTU richness, diversity and relative rarity. The eudominant bacteria in the guts and abdomens of bryophages were Novosphingobium, Bradyrhizobium, Ralstonia and Caulobacter, which are responsible for the detoxification of secondary metabolites or nitrogen fixation. These are less common in the surface samples and, therefore, likely to be associated with the specific ability of bryophages to feed on mosses.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnologické a biomedicínské centrum Akademie věd a Univerzity Karlovy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Entomology (on-line)

ISSN

1802-8829

e-ISSN

—

Svazek periodika

116

Číslo periodika v rámci svazku

November

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

432-441

Kód UT WoS článku

000545759600001

EID výsledku v databázi Scopus

2-s2.0-85083481013