Complexation of DNA with QPDMAEMA-b-PLMA-b-POEGMA Cationic Triblock Terpolymer Micelles
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10419519" target="_blank" >RIV/00216208:11310/20:10419519 - isvavai.cz</a>
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=0isX1y.MWX" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=0isX1y.MWX</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.macromol.0c00388" target="_blank" >10.1021/acs.macromol.0c00388</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Complexation of DNA with QPDMAEMA-b-PLMA-b-POEGMA Cationic Triblock Terpolymer Micelles
Popis výsledku v původním jazyce
In this work, we investigate the formation and structure of complexes between quaternized poly(2-(dimethylamino)ethyl methacrylate-b-lauryl methacrylate-b-(oligo ethylene glycol) methacrylate) (QPDMAEMA-b-PLMA-b-POEGMA) amphiphilic triblock terpolymer micelles, with different compositions, and DNAs of different lengths. In aqueous solutions, the terpolymers self-assemble in spherical micelles comprising hydrophobic PLMA cores and mixed QPDMAEMA/POEGMA coronas. Terpolymer/DNA micelleplexes were prepared in a wide range of amine over phosphate group ratios (N/P), and linear DNA of different lengths (2000 base pairs and 113 base pairs) was utilized. The complexation ability of the polymeric micelles with DNA was studied by UV-vis spectroscopy and ethidium bromide quenching assays using fluorescence spectroscopy. The thermodynamic interactions that take place during complexation were investigated by isothermal titration calorimetry, whereas the structural characteristics of the micelleplexes were determined by lights-cattering techniques (dynamic, static, and electrophoretic) and cryogenic transmission electron microscopy (cryo-TEM) imaging. All terpolymer micelles effectively complex DNAs, forming micelleplexes, with their size, surface charge, and structure depending on the ratio of the components, length of the DNA, and the chemical structure of the terpolymers. Cryo-TEM revealed the clustering of terpolymer micelles due to complexation with the clusters having different topologies influenced remarkably by the length of the DNA utilized.
Název v anglickém jazyce
Complexation of DNA with QPDMAEMA-b-PLMA-b-POEGMA Cationic Triblock Terpolymer Micelles
Popis výsledku anglicky
In this work, we investigate the formation and structure of complexes between quaternized poly(2-(dimethylamino)ethyl methacrylate-b-lauryl methacrylate-b-(oligo ethylene glycol) methacrylate) (QPDMAEMA-b-PLMA-b-POEGMA) amphiphilic triblock terpolymer micelles, with different compositions, and DNAs of different lengths. In aqueous solutions, the terpolymers self-assemble in spherical micelles comprising hydrophobic PLMA cores and mixed QPDMAEMA/POEGMA coronas. Terpolymer/DNA micelleplexes were prepared in a wide range of amine over phosphate group ratios (N/P), and linear DNA of different lengths (2000 base pairs and 113 base pairs) was utilized. The complexation ability of the polymeric micelles with DNA was studied by UV-vis spectroscopy and ethidium bromide quenching assays using fluorescence spectroscopy. The thermodynamic interactions that take place during complexation were investigated by isothermal titration calorimetry, whereas the structural characteristics of the micelleplexes were determined by lights-cattering techniques (dynamic, static, and electrophoretic) and cryogenic transmission electron microscopy (cryo-TEM) imaging. All terpolymer micelles effectively complex DNAs, forming micelleplexes, with their size, surface charge, and structure depending on the ratio of the components, length of the DNA, and the chemical structure of the terpolymers. Cryo-TEM revealed the clustering of terpolymer micelles due to complexation with the clusters having different topologies influenced remarkably by the length of the DNA utilized.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10404 - Polymer science
Návaznosti výsledku
Projekt
<a href="/cs/project/LTAIN19078" target="_blank" >LTAIN19078: Nanostrukturované micely s jedním či více kompartmenty a odezvou na vnější podněty tvořené blokovými kopolymery: Materiály a aplikace</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Macromolecules
ISSN
0024-9297
e-ISSN
—
Svazek periodika
53
Číslo periodika v rámci svazku
14
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
5747-5755
Kód UT WoS článku
000557761800010
EID výsledku v databázi Scopus
2-s2.0-85088022590