Electroanalytical methods for determination of 7-dehydrocholesterol in artificial serum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10420322" target="_blank" >RIV/00216208:11310/20:10420322 - isvavai.cz</a>

Výsledek na webu

<a href="http://web.natur.cuni.cz/analchem/isc/isc16.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc16.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electroanalytical methods for determination of 7-dehydrocholesterol in artificial serum

Popis výsledku v původním jazyce

7-Dehydrocholesterol is a biomarker of Smith-Lemli-Opitz syndrome, an autosomal recessive genetic disorder, caused by the inborn deficiency of 7-dehydrocholesterol reductase. In this study, procedures for its determination in artificial serum using flow injection analysis with electrochemical detection and voltammetric detection on boron doped diamond electrode were optimized. The proteins were precipitated by acetonitrile and after centrifugation the supernatant used for analysis. For quantitation of 7-DHC by differential pulse voltammetry the optimal ratio acetonitrile-artificial serum 9:1 (v/v) was applied. In FIA-ED, the ratio 3:1 (v/v), run electrolyte consisting of water-acetonitrile containing 0.01 mol L-1 NaClO in the same ratio 4 and detection potential of +1.3 V vs. Ag/AgCl (3 mol L-1 KCl) were used. Quantitation of 7-DHC was possible using calibration depen- dence with limit detection of 20 μmol L-1 in artificial serum. Nevertheless, the method has low recovery and for sensitive determination in real matrices of human serum and amniotic fluid a liquid-liquid extraction needs to be applied to prevent presence of 7-dehydrocholesterol in the phase with precipitated proteins.

Název v anglickém jazyce

Electroanalytical methods for determination of 7-dehydrocholesterol in artificial serum

Popis výsledku anglicky

7-Dehydrocholesterol is a biomarker of Smith-Lemli-Opitz syndrome, an autosomal recessive genetic disorder, caused by the inborn deficiency of 7-dehydrocholesterol reductase. In this study, procedures for its determination in artificial serum using flow injection analysis with electrochemical detection and voltammetric detection on boron doped diamond electrode were optimized. The proteins were precipitated by acetonitrile and after centrifugation the supernatant used for analysis. For quantitation of 7-DHC by differential pulse voltammetry the optimal ratio acetonitrile-artificial serum 9:1 (v/v) was applied. In FIA-ED, the ratio 3:1 (v/v), run electrolyte consisting of water-acetonitrile containing 0.01 mol L-1 NaClO in the same ratio 4 and detection potential of +1.3 V vs. Ag/AgCl (3 mol L-1 KCl) were used. Quantitation of 7-DHC was possible using calibration depen- dence with limit detection of 20 μmol L-1 in artificial serum. Nevertheless, the method has low recovery and for sensitive determination in real matrices of human serum and amniotic fluid a liquid-liquid extraction needs to be applied to prevent presence of 7-dehydrocholesterol in the phase with precipitated proteins.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA19-11268S" target="_blank" >GA19-11268S: Elektrochemické metody: Nové přístupy pro charakterizaci a analýzu steroidů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 16th International Students Conference &quot;Modern Analytical Chemistry&quot;

ISBN

978-80-7444-079-3

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

146-150

Název nakladatele

Univerzita Karlova, Přírodovědecká fakulta

Místo vydání

Praha

Místo konání akce

Prague

Datum konání akce

17. 9. 2020

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000623101400025