Sorting the Muck from the Brass: Analysis of Protein Complexes and Cell Lysates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10422672" target="_blank" >RIV/00216208:11310/20:10422672 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/978-1-0716-0294-2_38" target="_blank" >https://doi.org/10.1007/978-1-0716-0294-2_38</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-0294-2_38" target="_blank" >10.1007/978-1-0716-0294-2_38</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sorting the Muck from the Brass: Analysis of Protein Complexes and Cell Lysates

Popis výsledku v původním jazyce

Reliable determination of protein complex composition or changes to protein levels in whole cells is challenging. Despite the multitude of methods now available for labeling, analysis, and the statistical processing of data, this large variety is of itself an issue: Which approach is most appropriate, where do you set cutoffs, and what is the most cost-effective strategy? One size does not fit all for such work, but some guidelines can help in terms of reducing cost, improving data quality, and ultimately advancing investigations. Here we describe two protocols and algorithms for facile sample preparation for mass spectrometric analysis, robust data processing, and considerations of how to interpret large proteomic datasets in a productive and robust manner. (C) Springer Science+Business Media, LLC, part of Springer Nature 2020.

Název v anglickém jazyce

Sorting the Muck from the Brass: Analysis of Protein Complexes and Cell Lysates

Popis výsledku anglicky

Reliable determination of protein complex composition or changes to protein levels in whole cells is challenging. Despite the multitude of methods now available for labeling, analysis, and the statistical processing of data, this large variety is of itself an issue: Which approach is most appropriate, where do you set cutoffs, and what is the most cost-effective strategy? One size does not fit all for such work, but some guidelines can help in terms of reducing cost, improving data quality, and ultimately advancing investigations. Here we describe two protocols and algorithms for facile sample preparation for mass spectrometric analysis, robust data processing, and considerations of how to interpret large proteomic datasets in a productive and robust manner. (C) Springer Science+Business Media, LLC, part of Springer Nature 2020.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

—

Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Trypanosomatids: Methods and Protocols,

ISBN

978-1-07-160294-2

Počet stran výsledku

9

Strana od-do

645-653

Počet stran knihy

844

Název nakladatele

Springer

Místo vydání

New York : Humana Press. copyright 2020

Kód UT WoS kapitoly

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