Analysis of Mitochondrial Retrograde Signaling in Yeast Model Systems
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10428652" target="_blank" >RIV/00216208:11310/21:10428652 - isvavai.cz</a>
Výsledek na webu
<a href="https://link.springer.com/protocol/10.1007%2F978-1-0716-1266-8_6#citeas" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-0716-1266-8_6#citeas</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-1266-8_6" target="_blank" >10.1007/978-1-0716-1266-8_6</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Analysis of Mitochondrial Retrograde Signaling in Yeast Model Systems
Popis výsledku v původním jazyce
Mitochondrial retrograde signaling is a mitochondria-to-nucleus communication pathway, conserved from yeast to humans, by which dysfunctional mitochondria relay signals that lead to cell stress adaptation in physiopathological conditions via changes in nuclear gene expression. The most comprehensive picture of components and regulation of retrograde signaling has been obtained in Saccharomyces cerevisiae, where retrograde-target gene expression is regulated by RTG genes. In this chapter, we describe methods to measure mitochondrial retrograde pathway activation at the level of mRNA and protein products in yeast model systems, including cell suspensions and microcolonies. In particular, we will focus on three major procedures: mRNA levels of RTG-target genes, such as those encoding for peroxisomal citrate synthase (CIT2), aconitase, and NAD+-specific isocitrate dehydrogenase subunit 1 by real-time PCR; expression analysis of CIT2-gene protein product (Cit2p-GFP) by Western blot and fluorescence microscopy; the phosphorylation status of transcriptional factor Rtg1/3p which controls RTG-target gene transcription.
Název v anglickém jazyce
Analysis of Mitochondrial Retrograde Signaling in Yeast Model Systems
Popis výsledku anglicky
Mitochondrial retrograde signaling is a mitochondria-to-nucleus communication pathway, conserved from yeast to humans, by which dysfunctional mitochondria relay signals that lead to cell stress adaptation in physiopathological conditions via changes in nuclear gene expression. The most comprehensive picture of components and regulation of retrograde signaling has been obtained in Saccharomyces cerevisiae, where retrograde-target gene expression is regulated by RTG genes. In this chapter, we describe methods to measure mitochondrial retrograde pathway activation at the level of mRNA and protein products in yeast model systems, including cell suspensions and microcolonies. In particular, we will focus on three major procedures: mRNA levels of RTG-target genes, such as those encoding for peroxisomal citrate synthase (CIT2), aconitase, and NAD+-specific isocitrate dehydrogenase subunit 1 by real-time PCR; expression analysis of CIT2-gene protein product (Cit2p-GFP) by Western blot and fluorescence microscopy; the phosphorylation status of transcriptional factor Rtg1/3p which controls RTG-target gene transcription.
Klasifikace
Druh
C - Kapitola v odborné knize
CEP obor
—
OECD FORD obor
10600 - Biological sciences
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název knihy nebo sborníku
Mitochondrial Medicine, Volume 2: Assessing Mitochondria
ISBN
978-1-07-161265-1
Počet stran výsledku
16
Strana od-do
87-102
Počet stran knihy
459
Název nakladatele
Springer
Místo vydání
New York
Kód UT WoS kapitoly
—