AFB1 controls rapid auxin signalling through membrane depolarization in Arabidopsis thaliana root

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10432709" target="_blank" >RIV/00216208:11310/21:10432709 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/21:43922975 RIV/49777513:23640/21:43962550

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=_AVPrO89M5" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=_AVPrO89M5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41477-021-00969-z" target="_blank" >10.1038/s41477-021-00969-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

AFB1 controls rapid auxin signalling through membrane depolarization in Arabidopsis thaliana root

Popis výsledku v původním jazyce

The membrane potential reflects the difference between cytoplasmic and apoplastic electrical potentials and is essential for cellular operation. The application of the phytohormone auxin (3-indoleacetic acid (IAA)) causes instantaneous membrane depolarization in various cell types(1-6), making depolarization a hallmark of IAA-induced rapid responses. In root hairs, depolarization requires functional IAA transport and TIR1-AFB signalling(5), but its physiological importance is not understood. Specifically in roots, auxin triggers rapid growth inhibition(7-9) (RGI), a process required for gravitropic bending. RGI is initiated by the TIR1-AFB co-receptors, with the AFB1 paralogue playing a crucial role(10,11). The nature of the underlying rapid signalling is unknown, as well as the molecular machinery executing it. Even though the growth and depolarization responses to auxin show remarkable similarities, the importance of membrane depolarization for root growth inhibition and gravitropism is unclear. Here, by combining the DISBAC(2)(3) voltage sensor with microfluidics and vertical-stage microscopy, we show that rapid auxin-induced membrane depolarization tightly correlates with RGI. Rapid depolarization and RGI require the AFB1 auxin co-receptor. Finally, AFB1 is essential for the rapid formation of the membrane depolarization gradient across the gravistimulated root. These results clarify the role of AFB1 as the central receptor for rapid auxin responses.

Název v anglickém jazyce

AFB1 controls rapid auxin signalling through membrane depolarization in Arabidopsis thaliana root

Popis výsledku anglicky

The membrane potential reflects the difference between cytoplasmic and apoplastic electrical potentials and is essential for cellular operation. The application of the phytohormone auxin (3-indoleacetic acid (IAA)) causes instantaneous membrane depolarization in various cell types(1-6), making depolarization a hallmark of IAA-induced rapid responses. In root hairs, depolarization requires functional IAA transport and TIR1-AFB signalling(5), but its physiological importance is not understood. Specifically in roots, auxin triggers rapid growth inhibition(7-9) (RGI), a process required for gravitropic bending. RGI is initiated by the TIR1-AFB co-receptors, with the AFB1 paralogue playing a crucial role(10,11). The nature of the underlying rapid signalling is unknown, as well as the molecular machinery executing it. Even though the growth and depolarization responses to auxin show remarkable similarities, the importance of membrane depolarization for root growth inhibition and gravitropism is unclear. Here, by combining the DISBAC(2)(3) voltage sensor with microfluidics and vertical-stage microscopy, we show that rapid auxin-induced membrane depolarization tightly correlates with RGI. Rapid depolarization and RGI require the AFB1 auxin co-receptor. Finally, AFB1 is essential for the rapid formation of the membrane depolarization gradient across the gravistimulated root. These results clarify the role of AFB1 as the central receptor for rapid auxin responses.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GJ18-10116Y" target="_blank" >GJ18-10116Y: Vizualizace fyziologických procesů růstu kořene a charakterizace molekulárních mechanismů řídících buněčný růst u Arabidopsis thaliana</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Plants

ISSN

2055-026X

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

1229-1238

Kód UT WoS článku

000674542500001

EID výsledku v databázi Scopus

2-s2.0-85110717687