Complete Life Cycle of Trypanosoma thomasbancrofti, an Avian Trypanosome Transmitted by Culicine Mosquitoes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10436724" target="_blank" >RIV/00216208:11310/21:10436724 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Xfdz-NGKj4" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Xfdz-NGKj4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms9102101" target="_blank" >10.3390/microorganisms9102101</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Complete Life Cycle of Trypanosoma thomasbancrofti, an Avian Trypanosome Transmitted by Culicine Mosquitoes

Popis výsledku v původním jazyce

Avian trypanosomes are cosmopolitan and common protozoan parasites of birds; nevertheless, knowledge of their life cycles and vectors remains incomplete. Mosquitoes have been confirmed as vectors of Trypanosoma culicavium and suggested as vectors of T. thomasbancrofti; however, transmission has been experimentally confirmed only for the former species. This study aims to confirm the experimental transmission of T. thomasbancrofti to birds and its localization in vectors. Culex pipiens were fed on blood using four strains of T. thomasbancrofti, isolated from vectors and avian hosts; all strains established infections, and three of them were able to develop high infection rates in mosquitoes. The infection rate of the culicine isolates was 5-28% for CUL15 and 48-81% for CUL98, 67-92% for isolate OF19 from hippoboscid fly, while the avian isolate PAS343 ranged between 48% and 92%, and heavy infections were detected in 90% of positive females. Contrary to T. culicavium, trypanosomes were localized in the hindgut, where they formed rosettes with the occurrence of free epimastigotes in the hindgut and midgut during late infections. Parasites occurred in urine droplets produced during mosquito prediuresis. Transmission to birds was achieved by the ingestion of mosquito guts containing trypanosomes and via the conjunctiva. Bird infection was proven by blood cultivation and xenodiagnosis; mature infections were present in the dissected guts of 24-26% of mosquitoes fed on infected birds. The prevalence of T. thomasbancrofti in vectors in nature and in avian populations is discussed in this paper. This study confirms the vectorial capacity of culicine mosquitoes for T. thomasbancrofti, a trypanosome related to T. avium, and suggests that prediuresis might be an effective mode of trypanosome transmission.

Název v anglickém jazyce

Complete Life Cycle of Trypanosoma thomasbancrofti, an Avian Trypanosome Transmitted by Culicine Mosquitoes

Popis výsledku anglicky

Avian trypanosomes are cosmopolitan and common protozoan parasites of birds; nevertheless, knowledge of their life cycles and vectors remains incomplete. Mosquitoes have been confirmed as vectors of Trypanosoma culicavium and suggested as vectors of T. thomasbancrofti; however, transmission has been experimentally confirmed only for the former species. This study aims to confirm the experimental transmission of T. thomasbancrofti to birds and its localization in vectors. Culex pipiens were fed on blood using four strains of T. thomasbancrofti, isolated from vectors and avian hosts; all strains established infections, and three of them were able to develop high infection rates in mosquitoes. The infection rate of the culicine isolates was 5-28% for CUL15 and 48-81% for CUL98, 67-92% for isolate OF19 from hippoboscid fly, while the avian isolate PAS343 ranged between 48% and 92%, and heavy infections were detected in 90% of positive females. Contrary to T. culicavium, trypanosomes were localized in the hindgut, where they formed rosettes with the occurrence of free epimastigotes in the hindgut and midgut during late infections. Parasites occurred in urine droplets produced during mosquito prediuresis. Transmission to birds was achieved by the ingestion of mosquito guts containing trypanosomes and via the conjunctiva. Bird infection was proven by blood cultivation and xenodiagnosis; mature infections were present in the dissected guts of 24-26% of mosquitoes fed on infected birds. The prevalence of T. thomasbancrofti in vectors in nature and in avian populations is discussed in this paper. This study confirms the vectorial capacity of culicine mosquitoes for T. thomasbancrofti, a trypanosome related to T. avium, and suggests that prediuresis might be an effective mode of trypanosome transmission.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microorganisms [online]

ISSN

2076-2607

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

2101

Kód UT WoS článku

000726389100001

EID výsledku v databázi Scopus

2-s2.0-85116203013