Analytical determination of oestrogenic endocrine disruptors: the method of choice for wastewater treatment plant effluents

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10437178" target="_blank" >RIV/00216208:11310/21:10437178 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/21:00547234

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=9V2Dqh0aL~" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=9V2Dqh0aL~</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1071/EN21028" target="_blank" >10.1071/EN21028</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analytical determination of oestrogenic endocrine disruptors: the method of choice for wastewater treatment plant effluents

Popis výsledku v původním jazyce

Two analytical approaches - liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS) methods - were compared for the simultaneous determination of the 19 most important oestrogenic endocrine disrupting chemicals (EDCs), such as 17 beta-oestradiol, oestrone, 17 alpha-ethinyloestradiol, bisphenol A and triclosan in wastewater treatment plant effluents. To lower the instrument limits of detection (ILODs), a derivatisation step preceded detection in both methods. The stability, sensitivity and ease of use of dansylation (Dns) for LC-MS/MS and trimethylsilylation (TMS) for GC-MS/MS derivatives were evaluated before method validation. TMS derivatisation products were highly unstable over time. Parameters such as susceptibility to matrix effects and the stability of monodansylated and didansylated derivatisation products of phytohormones are discussed. Lower ILODs of highly potent EDCs (0.11 ng mL(-1) for 17 beta-oestradiol, 0.01 ng mL(-1) for 17 alpha-ethinyloestradiol and 0.22 ng mL(-1) for oestrone) and stability of derivatisation products within 7 days were achieved using LC-MS/MS; therefore, further validation of this method at environmentally relevant concentrations was conducted. The method limits of detection (MLODs) met the requirements of the European Union defined in Directive 2008/105/ES for 17 alpha-ethinyloestradiol (0.035 ng L-1) and 17 beta-oestradiol (0.4 ng L-1). Twenty samples of wastewater treatment plant effluent from the Czech Republic were screened using LC-MS/MS. Fifteen of the EDCs were detected in at least one sample. The most abundant EDCs were bisphenol A, with a concentration up to 1107 ng L-1, and triclosan, with a concentration up to 76 ng L-1. No seasonal trend between late spring and autumn samples was observed in the frequency or quantity of analytes.

Název v anglickém jazyce

Analytical determination of oestrogenic endocrine disruptors: the method of choice for wastewater treatment plant effluents

Popis výsledku anglicky

Two analytical approaches - liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS) methods - were compared for the simultaneous determination of the 19 most important oestrogenic endocrine disrupting chemicals (EDCs), such as 17 beta-oestradiol, oestrone, 17 alpha-ethinyloestradiol, bisphenol A and triclosan in wastewater treatment plant effluents. To lower the instrument limits of detection (ILODs), a derivatisation step preceded detection in both methods. The stability, sensitivity and ease of use of dansylation (Dns) for LC-MS/MS and trimethylsilylation (TMS) for GC-MS/MS derivatives were evaluated before method validation. TMS derivatisation products were highly unstable over time. Parameters such as susceptibility to matrix effects and the stability of monodansylated and didansylated derivatisation products of phytohormones are discussed. Lower ILODs of highly potent EDCs (0.11 ng mL(-1) for 17 beta-oestradiol, 0.01 ng mL(-1) for 17 alpha-ethinyloestradiol and 0.22 ng mL(-1) for oestrone) and stability of derivatisation products within 7 days were achieved using LC-MS/MS; therefore, further validation of this method at environmentally relevant concentrations was conducted. The method limits of detection (MLODs) met the requirements of the European Union defined in Directive 2008/105/ES for 17 alpha-ethinyloestradiol (0.035 ng L-1) and 17 beta-oestradiol (0.4 ng L-1). Twenty samples of wastewater treatment plant effluent from the Czech Republic were screened using LC-MS/MS. Fifteen of the EDCs were detected in at least one sample. The most abundant EDCs were bisphenol A, with a concentration up to 1107 ng L-1, and triclosan, with a concentration up to 76 ng L-1. No seasonal trend between late spring and autumn samples was observed in the frequency or quantity of analytes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

<a href="/cs/project/SS02030008" target="_blank" >SS02030008: Centrum environmentálního výzkumu: Odpadové a oběhové hospodářství a environmentální bezpečnost</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Chemistry

ISSN

1448-2517

e-ISSN

—

Svazek periodika

18

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

AU - Austrálie

Počet stran výsledku

13

Strana od-do

143-155

Kód UT WoS článku

000673558800001

EID výsledku v databázi Scopus

2-s2.0-85110590794