Identification of sex chromosomes using genomic and cytogenetic methods in a range-expanding spider, Argiope bruennichi (Araneae: Araneidae)
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10444504" target="_blank" >RIV/00216208:11310/22:10444504 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14310/22:00126938
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Ms9Jrx13jh" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Ms9Jrx13jh</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/biolinnean/blac039" target="_blank" >10.1093/biolinnean/blac039</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Identification of sex chromosomes using genomic and cytogenetic methods in a range-expanding spider, Argiope bruennichi (Araneae: Araneidae)
Popis výsledku v původním jazyce
Differences between sexes in growth, ecology and behaviour strongly shape species biology. In some animal groups, such as spiders, it is difficult or impossible to identify the sex of juveniles based on external morphology. This information would be useful for field surveys, behavioural experiments and ecological studies, such as those on sex ratios and dispersal. In species with sex chromosomes, sex can be determined based on the specific sex chromosome complement. Additionally, information on the sequence of sex chromosomes provides the basis for studying sex chromosome evolution. We combined cytogenetic and genomic data to identify the sex chromosomes in the sexually dimorphic spider Argiope bruennichi and designed quantitative real-time polymerase chain reaction sex markers. We found that the genome size and GC content of this spider fall into the ranges reported for the majority of araneids. The male karyotype is formed by 24 acrocentric chromosomes with an X(1)X(2)0 sex chromosome system, with little similarity between X chromosomes, suggesting an origin of these chromosomes by X chromosome fission or early duplication of an X chromosome and subsequent independent differentiation of the copies. Our data suggest X chromosomes of similar sizes in A. bruennichi. They are smaller chromosomes of the complement. Our findings open the door to new directions in spider evolutionary and ecological research.
Název v anglickém jazyce
Identification of sex chromosomes using genomic and cytogenetic methods in a range-expanding spider, Argiope bruennichi (Araneae: Araneidae)
Popis výsledku anglicky
Differences between sexes in growth, ecology and behaviour strongly shape species biology. In some animal groups, such as spiders, it is difficult or impossible to identify the sex of juveniles based on external morphology. This information would be useful for field surveys, behavioural experiments and ecological studies, such as those on sex ratios and dispersal. In species with sex chromosomes, sex can be determined based on the specific sex chromosome complement. Additionally, information on the sequence of sex chromosomes provides the basis for studying sex chromosome evolution. We combined cytogenetic and genomic data to identify the sex chromosomes in the sexually dimorphic spider Argiope bruennichi and designed quantitative real-time polymerase chain reaction sex markers. We found that the genome size and GC content of this spider fall into the ranges reported for the majority of araneids. The male karyotype is formed by 24 acrocentric chromosomes with an X(1)X(2)0 sex chromosome system, with little similarity between X chromosomes, suggesting an origin of these chromosomes by X chromosome fission or early duplication of an X chromosome and subsequent independent differentiation of the copies. Our data suggest X chromosomes of similar sizes in A. bruennichi. They are smaller chromosomes of the complement. Our findings open the door to new directions in spider evolutionary and ecological research.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10600 - Biological sciences
Návaznosti výsledku
Projekt
<a href="/cs/project/LTAUSA19142" target="_blank" >LTAUSA19142: Evoluce karyotypu, pohlavních chromozomů a genomu ve fylogeneticky bazálních větvích araneomorfních pavouků</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biological Journal of the Linnean Society
ISSN
0024-4066
e-ISSN
1095-8312
Svazek periodika
136
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
12
Strana od-do
405-416
Kód UT WoS článku
000800203100001
EID výsledku v databázi Scopus
2-s2.0-85132102824