Linear dicarboxylato and tridentate chelating ligands coordinated Cu(II) complexes: Syntheses, crystal structures, protein binding and cytotoxicity studies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10444994" target="_blank" >RIV/00216208:11310/22:10444994 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=E~Nwox_b_i" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=E~Nwox_b_i</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.poly.2022.115888" target="_blank" >10.1016/j.poly.2022.115888</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Linear dicarboxylato and tridentate chelating ligands coordinated Cu(II) complexes: Syntheses, crystal structures, protein binding and cytotoxicity studies

Popis výsledku v původním jazyce

Three dinuclear copper(II) complexes, [Cu(2)(L1)(2)(tp)] . 4H2O (1), [Cu(2)(L1)(2)(ppda)(H2O)(2)] . 2H2O (2) and [Cu(2)(L2)(2)(tp)(CH3OH)(2)] . 2H2O (3) (where HL1 = 2-methoxy-6-[(2-morpholin-4-yl-ethylimino)-methyl]-phenol, HL2 = 2-ethoxy-6-[(2-morpholin-4-yl-ethylimino)-methyl]-phenol, tp = terephthalate, ppda = p-phenyl-enediacrylate), have been synthesized and characterized by single crystal X-ray diffraction, FTIR, mass spectrometry and thermal analysis. All the complexes crystallize in the monoclinic system with the space group P2(1)/n (for 1 and 2) and P2(1)/c (for 3) as centrosymmetric species. In 1 and 3, two CuL1 and CuL2 units, respectively are bridged by a terephthalate (tp) anion, whereas in 2 the bridging unit is p-phenylenediacrylate (ppda). The copper atoms in 1 exhibit a square planar coordination geometry, while in 2 and 3 the metals have a square pyramidal environment. All of 1-3 form 1D supramolecular chains through C-H...pi interaction. The interaction of the complexes with bovine serum albumin (BSA) was investigated using a fluorescence spectroscopic technique. The values of the binding constant are (15.9 +/- 0.13) x 10(4) M(-1) (for 1), (10.3 +/- 0.08) x 10(4) M(-1) (for 2) and (11.2 +/- 0.37) x 10(4) M(-1) (for 3). Using the Scatchard equation, the calculated values of the number of binding sites (n) are all around 1, signifying the presence of a single binding site in serum albumins for the Cu(II) complexes. The cytotoxicity of the complexes was tested using breast cancer MCF7 and MDA-MB-231 cells, along with normal breast epithelial MCF10A cells. MTT results reveal that the complexes potently suppressed the growth of the breast cancer cell lines MCF7 and MDA-MB-231, and the MCF10A cells. Complexes 1-3 inhibited fifty percent growth (IC50) at 5.80 +/- 1.47, 20.85 +/- 1.25 and 5.10 +/- 1.23 μM for MCF7 cells, respectively. Treatment of complex 3 results in perturbation of the microtubule organization, which might be through attenuation of the tubulin level.

Název v anglickém jazyce

Linear dicarboxylato and tridentate chelating ligands coordinated Cu(II) complexes: Syntheses, crystal structures, protein binding and cytotoxicity studies

Popis výsledku anglicky

Three dinuclear copper(II) complexes, [Cu(2)(L1)(2)(tp)] . 4H2O (1), [Cu(2)(L1)(2)(ppda)(H2O)(2)] . 2H2O (2) and [Cu(2)(L2)(2)(tp)(CH3OH)(2)] . 2H2O (3) (where HL1 = 2-methoxy-6-[(2-morpholin-4-yl-ethylimino)-methyl]-phenol, HL2 = 2-ethoxy-6-[(2-morpholin-4-yl-ethylimino)-methyl]-phenol, tp = terephthalate, ppda = p-phenyl-enediacrylate), have been synthesized and characterized by single crystal X-ray diffraction, FTIR, mass spectrometry and thermal analysis. All the complexes crystallize in the monoclinic system with the space group P2(1)/n (for 1 and 2) and P2(1)/c (for 3) as centrosymmetric species. In 1 and 3, two CuL1 and CuL2 units, respectively are bridged by a terephthalate (tp) anion, whereas in 2 the bridging unit is p-phenylenediacrylate (ppda). The copper atoms in 1 exhibit a square planar coordination geometry, while in 2 and 3 the metals have a square pyramidal environment. All of 1-3 form 1D supramolecular chains through C-H...pi interaction. The interaction of the complexes with bovine serum albumin (BSA) was investigated using a fluorescence spectroscopic technique. The values of the binding constant are (15.9 +/- 0.13) x 10(4) M(-1) (for 1), (10.3 +/- 0.08) x 10(4) M(-1) (for 2) and (11.2 +/- 0.37) x 10(4) M(-1) (for 3). Using the Scatchard equation, the calculated values of the number of binding sites (n) are all around 1, signifying the presence of a single binding site in serum albumins for the Cu(II) complexes. The cytotoxicity of the complexes was tested using breast cancer MCF7 and MDA-MB-231 cells, along with normal breast epithelial MCF10A cells. MTT results reveal that the complexes potently suppressed the growth of the breast cancer cell lines MCF7 and MDA-MB-231, and the MCF10A cells. Complexes 1-3 inhibited fifty percent growth (IC50) at 5.80 +/- 1.47, 20.85 +/- 1.25 and 5.10 +/- 1.23 μM for MCF7 cells, respectively. Treatment of complex 3 results in perturbation of the microtubule organization, which might be through attenuation of the tubulin level.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10402 - Inorganic and nuclear chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Polyhedron

ISSN

0277-5387

e-ISSN

1873-3719

Svazek periodika

222

Číslo periodika v rámci svazku

August

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

115888

Kód UT WoS článku

000812200100001

EID výsledku v databázi Scopus

2-s2.0-85130323535