New strategy in electrochemical investigation of DNA damage demonstrated on genotoxic derivatives of fluorene

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10445189" target="_blank" >RIV/00216208:11310/22:10445189 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=6wZuh9B4HQ" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=6wZuh9B4HQ</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2022.116430" target="_blank" >10.1016/j.jelechem.2022.116430</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New strategy in electrochemical investigation of DNA damage demonstrated on genotoxic derivatives of fluorene

Popis výsledku v původním jazyce

In this work, a simple and fast electrochemical DNA biosensor based on a glassy carbon electrode (GCE) was prepared by adsorbing double-stranded DNA (dsDNA) from salmon sperm as a biorecognition layer onto the GCE surface. The dsDNA/GCE biosensor was characterized using modern imaging techniques. The prepared dsDNA/GCE biosensor was used for detection of dsDNA damage by 2-aminofluorene (2-AF), its metabolite 2-acetylaminofluorene (2-AAF), and 2,7-diaminofluorene (2,7-DAF). The electrochemical investigation of the interaction between the selected analytes and dsDNA was conducted using a combination of several electrochemical detection techniques (representing a comprehensive electrochemical biosensing approach allowing an overall assessment of dsDNA damage, even when the intrinsic voltammetric signals of the studied analytes overlap with the dsDNA signals): (i) cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) at the dsDNA/GCE biosensor, and (ii) differential pulse voltammetry (DPV) for measurements directly in solution at the bare GCE. The changes in dsDNA structure caused by structurally similar analytes were observed. The mutual interaction between derivatives of fluorene and dsDNA caused damaging effects of the test substances on the dsDNA structure in vitro.

Název v anglickém jazyce

New strategy in electrochemical investigation of DNA damage demonstrated on genotoxic derivatives of fluorene

Popis výsledku anglicky

In this work, a simple and fast electrochemical DNA biosensor based on a glassy carbon electrode (GCE) was prepared by adsorbing double-stranded DNA (dsDNA) from salmon sperm as a biorecognition layer onto the GCE surface. The dsDNA/GCE biosensor was characterized using modern imaging techniques. The prepared dsDNA/GCE biosensor was used for detection of dsDNA damage by 2-aminofluorene (2-AF), its metabolite 2-acetylaminofluorene (2-AAF), and 2,7-diaminofluorene (2,7-DAF). The electrochemical investigation of the interaction between the selected analytes and dsDNA was conducted using a combination of several electrochemical detection techniques (representing a comprehensive electrochemical biosensing approach allowing an overall assessment of dsDNA damage, even when the intrinsic voltammetric signals of the studied analytes overlap with the dsDNA signals): (i) cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) at the dsDNA/GCE biosensor, and (ii) differential pulse voltammetry (DPV) for measurements directly in solution at the bare GCE. The changes in dsDNA structure caused by structurally similar analytes were observed. The mutual interaction between derivatives of fluorene and dsDNA caused damaging effects of the test substances on the dsDNA structure in vitro.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA20-01589S" target="_blank" >GA20-01589S: Nové strategie pro zlepšení senzorových vlastností nových elektrodových materiálů prostřednictvím jejich předúpravy či modifikace povrchu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

1873-2569

Svazek periodika

918

Číslo periodika v rámci svazku

August

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

116430

Kód UT WoS článku

000817795100007

EID výsledku v databázi Scopus

2-s2.0-85131958610