Expression of αV integrin and its potential partners in bull reproductive tissues, germ cells and spermatozoa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10447037" target="_blank" >RIV/00216208:11310/22:10447037 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=PUivwqIj6B" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=PUivwqIj6B</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2022.04.001" target="_blank" >10.1016/j.ijbiomac.2022.04.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression of αV integrin and its potential partners in bull reproductive tissues, germ cells and spermatozoa

Popis výsledku v původním jazyce

Integrins are transmembrane receptors expressed in all nucleated mammalian cells, critically involved in cell-matrix adhesion and cell-cell interactions that modulate many signalling cascades. It is assumed that integrins also provide essential functions of the reproductive system. In this study, we describe the detailed localization and distribution of αV integrin in the plasma membrane of bull sperm head and tail. Integrin αV was observed in the area of forming acrosome in developing sperm since the stage of round spermatids and persists in the acrosome during epididymal maturation and ejaculation till the acrosomal exocytosis. We detected CD9 and CD81 tetraspanins as the potential partners of αV integrin. Their similar staining pattern in testicular tissue suggested the involvement of these molecules in the tetraspanin web of &quot;testisomes&quot;. Moreover, the complex of αV with β1 and β3 integrin subunits cannot be excluded at least in sperm. The presented findings contribute to understanding the mutual action of integrins and tetraspanins during sperm development and maturation.

Název v anglickém jazyce

Expression of αV integrin and its potential partners in bull reproductive tissues, germ cells and spermatozoa

Popis výsledku anglicky

Integrins are transmembrane receptors expressed in all nucleated mammalian cells, critically involved in cell-matrix adhesion and cell-cell interactions that modulate many signalling cascades. It is assumed that integrins also provide essential functions of the reproductive system. In this study, we describe the detailed localization and distribution of αV integrin in the plasma membrane of bull sperm head and tail. Integrin αV was observed in the area of forming acrosome in developing sperm since the stage of round spermatids and persists in the acrosome during epididymal maturation and ejaculation till the acrosomal exocytosis. We detected CD9 and CD81 tetraspanins as the potential partners of αV integrin. Their similar staining pattern in testicular tissue suggested the involvement of these molecules in the tetraspanin web of &quot;testisomes&quot;. Moreover, the complex of αV with β1 and β3 integrin subunits cannot be excluded at least in sperm. The presented findings contribute to understanding the mutual action of integrins and tetraspanins during sperm development and maturation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

1879-0003

Svazek periodika

209

Číslo periodika v rámci svazku

Part A

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

542-551

Kód UT WoS článku

000919077200001

EID výsledku v databázi Scopus

2-s2.0-85128190375