Improvement of conditions for the determination of neurotransmitters in rat brain tissue by HPLC with fluorimetric detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10452864" target="_blank" >RIV/00216208:11310/22:10452864 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=q13J3.LJ75" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=q13J3.LJ75</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-022-02924-w" target="_blank" >10.1007/s00706-022-02924-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Improvement of conditions for the determination of neurotransmitters in rat brain tissue by HPLC with fluorimetric detection

Popis výsledku v původním jazyce

Gradient elution for the determination of six selected neurotransmitters (3,4-dihydroxyphenylacetic acid, dopamine, homovanillic acid, 5-hydroxyindole-3-acetic acid, L-noradrenaline, serotonin) by HPLC with fluorescence detection on the C18 reverse phase was designed and optimized. The elution used the binary mobile phase of aqueous solution of 12x10(-3) mol dm(-3) acetic acid, 0.26x10(-3) mol dm(-3) Na2EDTA, pH = 3.5) and methanol. Furthermore, modified homogenization medium, prepared by mixing methanol and an aqueous solution (0.4 mol dm(-3) perchloric acid, 6x10(-3) mol dm-3 cysteine, 1x10(-3) mol dm(-3) L-ascorbic acid, and 0.54x10(-3) mol dm(-3) Na2EDTA) in a ratio of 1:1 (v/v), was introduced in the preparation of rat brain tissue to increase the stability of neurotransmitters in the sample before the analysis. Both developed procedures were successfully applied to the determination of selected neurotransmitters in authentic brain tissue samples from rats affected by morphine and/or morphine abstinence. The statistically significant increases in dopamine, 3,4-dihydroxyphenylacetic acid, and serotonin levels in the striatum, as well as L-noradrenaline in the cerebral cortex of rats affected by intraperitoneal administration of morphine, were measured.

Název v anglickém jazyce

Improvement of conditions for the determination of neurotransmitters in rat brain tissue by HPLC with fluorimetric detection

Popis výsledku anglicky

Gradient elution for the determination of six selected neurotransmitters (3,4-dihydroxyphenylacetic acid, dopamine, homovanillic acid, 5-hydroxyindole-3-acetic acid, L-noradrenaline, serotonin) by HPLC with fluorescence detection on the C18 reverse phase was designed and optimized. The elution used the binary mobile phase of aqueous solution of 12x10(-3) mol dm(-3) acetic acid, 0.26x10(-3) mol dm(-3) Na2EDTA, pH = 3.5) and methanol. Furthermore, modified homogenization medium, prepared by mixing methanol and an aqueous solution (0.4 mol dm(-3) perchloric acid, 6x10(-3) mol dm-3 cysteine, 1x10(-3) mol dm(-3) L-ascorbic acid, and 0.54x10(-3) mol dm(-3) Na2EDTA) in a ratio of 1:1 (v/v), was introduced in the preparation of rat brain tissue to increase the stability of neurotransmitters in the sample before the analysis. Both developed procedures were successfully applied to the determination of selected neurotransmitters in authentic brain tissue samples from rats affected by morphine and/or morphine abstinence. The statistically significant increases in dopamine, 3,4-dihydroxyphenylacetic acid, and serotonin levels in the striatum, as well as L-noradrenaline in the cerebral cortex of rats affected by intraperitoneal administration of morphine, were measured.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA19-03295S" target="_blank" >GA19-03295S: Důsledky dlouhodobého podávání a vysazení morfinu na mozek potkana: proteomické a funkční studie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

1434-4475

Svazek periodika

153

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

6

Strana od-do

753-758

Kód UT WoS článku

000796806900002

EID výsledku v databázi Scopus

2-s2.0-85130479163