Electrochemical behavior of methylene blue at bare and DNA-modified silver solid amalgam electrodes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10454779" target="_blank" >RIV/00216208:11310/22:10454779 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=8jwOyDjxkw" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=8jwOyDjxkw</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10008-022-05270-3" target="_blank" >10.1007/s10008-022-05270-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical behavior of methylene blue at bare and DNA-modified silver solid amalgam electrodes

Popis výsledku v původním jazyce

Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used for a voltammetric study of methylene blue (MB) at a mercury meniscus-modified silver solid amalgam electrode (m-AgSAE). Electrochemical impedance spectroscopy (EIS) was used for the verification and comparison of results. DPV offered sensitive detection of MB (in 0.1 mol L(-1) phosphate buffer of pH 7.0) at both a bare and a DNA-modified silver amalgam electrode. MB gives a specific cathodic signal related to the reduction of this redox intercalator. This signal is significantly higher at the DNA-modified than at the unmodified silver amalgam electrode, which is in correspondence with the changes in charge transfer resistance values obtained from Nyquist plots. The concentration of double-stranded DNA (dsDNA) to form a layer (0.1 mg mL(-1)), the time of the dsDNA spontaneous immobilization on the surface of the electrode (1 min), the concentration of MB (1x10(-5) mol L(-1)), and the time of the accumulation of MB into the dsDNA layer (1 min) were optimized for further biosensor development and applications.

Název v anglickém jazyce

Electrochemical behavior of methylene blue at bare and DNA-modified silver solid amalgam electrodes

Popis výsledku anglicky

Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used for a voltammetric study of methylene blue (MB) at a mercury meniscus-modified silver solid amalgam electrode (m-AgSAE). Electrochemical impedance spectroscopy (EIS) was used for the verification and comparison of results. DPV offered sensitive detection of MB (in 0.1 mol L(-1) phosphate buffer of pH 7.0) at both a bare and a DNA-modified silver amalgam electrode. MB gives a specific cathodic signal related to the reduction of this redox intercalator. This signal is significantly higher at the DNA-modified than at the unmodified silver amalgam electrode, which is in correspondence with the changes in charge transfer resistance values obtained from Nyquist plots. The concentration of double-stranded DNA (dsDNA) to form a layer (0.1 mg mL(-1)), the time of the dsDNA spontaneous immobilization on the surface of the electrode (1 min), the concentration of MB (1x10(-5) mol L(-1)), and the time of the accumulation of MB into the dsDNA layer (1 min) were optimized for further biosensor development and applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA20-01589S" target="_blank" >GA20-01589S: Nové strategie pro zlepšení senzorových vlastností nových elektrodových materiálů prostřednictvím jejich předúpravy či modifikace povrchu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Solid State Electrochemistry

ISSN

1432-8488

e-ISSN

1433-0768

Svazek periodika

26

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

2491-2499

Kód UT WoS článku

000840615200002

EID výsledku v databázi Scopus

2-s2.0-85124261364