Relative DNA content differences reliably identify Solidago xniederederi, a hybrid between native and invasive alien species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10457559" target="_blank" >RIV/00216208:11310/22:10457559 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=q0MkEOEOcq" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=q0MkEOEOcq</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.23855/preslia.2022.183" target="_blank" >10.23855/preslia.2022.183</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Relative DNA content differences reliably identify Solidago xniederederi, a hybrid between native and invasive alien species

Popis výsledku v původním jazyce

Hybridization between native and alien congeners may pose a serious threat to biodiversity and negatively affect native flora. Here we study Solidago xniederederi, which originated and became established in Europe as a result of a cross between the alien S. canadensis and native S. virgaurea. The recent increase in the number of records of S. xniederederi in Europe has highlighted the need to monitor its occurrence, spread and behaviour. In the present study, we tested the effectiveness of flow cytometry for detecting hybrid plants of S. xniederederi. Sequences of the ITS region of nrDNA and the rpS15-ycf1 spacer of cpDNA were used to confirm the hybrid origin of analysed plants and to identify the maternal species. Our study included 60 single-species populations of S. canadensis, S. gigantea and S. virgaurea, and 16 mixed populations with the presence of hybrid S. xniederederi sampled from six countries in central Europe and adjacent areas. All individuals of S. canadensis, S. xniederederi and S. virgaurea investigated were diploid (2n similar to 2x similar to 18) but differed in their relative DNA content values. The DNA content of S. xniederederi was intermediate between S. canadensis and S. virgaurea with no overlaps, with the differences between the species being statistically significant. Therefore, we conclude that flow cytometry is a reliable and efficient method for detailed screening for hybrids within mixed Solidago populations and for identifying non-flowering or morphologically ambiguous Solidago plants. Since both parental species varied only negligibly in their DNA content, it may also be applicable across a broader geographic scale. Genetic, flow cytometric and distributional data suggest that the hybrids are to a large extent early generation (likely F1) hybrids as very few cases of supposed introgressants were also inferred. The results from chloroplast rpS15-ycf1 spacer showed that hybridization has occurred in both directions.

Název v anglickém jazyce

Relative DNA content differences reliably identify Solidago xniederederi, a hybrid between native and invasive alien species

Popis výsledku anglicky

Hybridization between native and alien congeners may pose a serious threat to biodiversity and negatively affect native flora. Here we study Solidago xniederederi, which originated and became established in Europe as a result of a cross between the alien S. canadensis and native S. virgaurea. The recent increase in the number of records of S. xniederederi in Europe has highlighted the need to monitor its occurrence, spread and behaviour. In the present study, we tested the effectiveness of flow cytometry for detecting hybrid plants of S. xniederederi. Sequences of the ITS region of nrDNA and the rpS15-ycf1 spacer of cpDNA were used to confirm the hybrid origin of analysed plants and to identify the maternal species. Our study included 60 single-species populations of S. canadensis, S. gigantea and S. virgaurea, and 16 mixed populations with the presence of hybrid S. xniederederi sampled from six countries in central Europe and adjacent areas. All individuals of S. canadensis, S. xniederederi and S. virgaurea investigated were diploid (2n similar to 2x similar to 18) but differed in their relative DNA content values. The DNA content of S. xniederederi was intermediate between S. canadensis and S. virgaurea with no overlaps, with the differences between the species being statistically significant. Therefore, we conclude that flow cytometry is a reliable and efficient method for detailed screening for hybrids within mixed Solidago populations and for identifying non-flowering or morphologically ambiguous Solidago plants. Since both parental species varied only negligibly in their DNA content, it may also be applicable across a broader geographic scale. Genetic, flow cytometric and distributional data suggest that the hybrids are to a large extent early generation (likely F1) hybrids as very few cases of supposed introgressants were also inferred. The results from chloroplast rpS15-ycf1 spacer showed that hybridization has occurred in both directions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Preslia

ISSN

0032-7786

e-ISSN

2570-950X

Svazek periodika

94

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

31

Strana od-do

183-213

Kód UT WoS článku

000789270500005

EID výsledku v databázi Scopus

2-s2.0-85127872922