HPLC method development for untargeted metabolomics of polar compounds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10458020" target="_blank" >RIV/00216208:11310/22:10458020 - isvavai.cz</a>

Výsledek na webu

<a href="http://web.natur.cuni.cz/analchem/isc/isc18.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc18.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

HPLC method development for untargeted metabolomics of polar compounds

Popis výsledku v původním jazyce

LC-MS-based metabolomics is a fast-spreading approach to study microorganisms and their metabolism. This work is focused on LC-MS metabolomics of polar compounds, which are not retained on chromatographic columns using the well-established reversed-phase mechanism. The HILIC and multimode columns are expected to improve the retention characteristics of polar compounds. In the experimental part, four HILIC columns and two mixed-mode columns were tested. The columns were compared with a CSH-C18 column, using a set of 258 polar, human and microbial metabolites with a high diversity of their physicochemical properties. All HILIC columns significantly extended the range of the CSH-C18 column. In terms of the number of compounds detected, the BEH Amide PEEK column using ammonium acetate pH = 3 had the highest ability to analyze a wide spectrum of polar metabolites. The combination of the mentioned results is promising for the use of this method in untargeted metabolomics.

Název v anglickém jazyce

HPLC method development for untargeted metabolomics of polar compounds

Popis výsledku anglicky

LC-MS-based metabolomics is a fast-spreading approach to study microorganisms and their metabolism. This work is focused on LC-MS metabolomics of polar compounds, which are not retained on chromatographic columns using the well-established reversed-phase mechanism. The HILIC and multimode columns are expected to improve the retention characteristics of polar compounds. In the experimental part, four HILIC columns and two mixed-mode columns were tested. The columns were compared with a CSH-C18 column, using a set of 258 polar, human and microbial metabolites with a high diversity of their physicochemical properties. All HILIC columns significantly extended the range of the CSH-C18 column. In terms of the number of compounds detected, the BEH Amide PEEK column using ammonium acetate pH = 3 had the highest ability to analyze a wide spectrum of polar metabolites. The combination of the mentioned results is promising for the use of this method in untargeted metabolomics.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 18th International Students Conference &quot;Modern Analytical Chemistry&quot;

ISBN

978-80-7444-093-9

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

7-12

Název nakladatele

Univerzita Karlova, Přírodovědecká fakulta

Místo vydání

Praha

Místo konání akce

Prague

Datum konání akce

15. 9. 2022

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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