Simultaneous detection and quantification of two European anglerfishes by novel genomic primer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10453733" target="_blank" >RIV/00216208:11310/23:10453733 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/22:43925110 RIV/00027022:_____/22:N0000070

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=vtqhXXw8PO" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=vtqhXXw8PO</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jfca.2022.104992" target="_blank" >10.1016/j.jfca.2022.104992</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous detection and quantification of two European anglerfishes by novel genomic primer

Popis výsledku v původním jazyce

Globalisation has led to increased trade and consumption of fish worldwide. As international trade and con-sumption of fish increases, so does the likelihood of fish being adulterated. It is often an illegal exchange of species where a cheaper species is substituted for a more expensive and rarer one. In Europe, the anglerfish (Lophius piscatorius) is often traded as the rarer, more popular black-bellied anglerfish (Lophius budegassa). To improve our ability to monitor and detect adulterants in these fish species, we developed a real-time PCR assay that allows for the simultaneous detection and quantification of L. piscatorius and L. budegassa. The newly designed primers target the second intron of the genomic gene parvalbumin. The proposed methodology shows good robustness, efficiency and high specificity. Of the 47 species tested, only Lophius species were amplified. Their differentiation is possible by analysing melting curves with an average Tm of 70.1 °C for L. piscatorius and 75.3 °C for L. budegassa, respectively. The detection limit for both species was 0.050 ng/μl. The assay also provides a tool to quantify parvalbumin in European anglerfish. The findings point to the use of the method proposed as a potential tool in forensic investigations to prevent illegal species substitutions, mislabeling, and food fraud.

Název v anglickém jazyce

Simultaneous detection and quantification of two European anglerfishes by novel genomic primer

Popis výsledku anglicky

Globalisation has led to increased trade and consumption of fish worldwide. As international trade and con-sumption of fish increases, so does the likelihood of fish being adulterated. It is often an illegal exchange of species where a cheaper species is substituted for a more expensive and rarer one. In Europe, the anglerfish (Lophius piscatorius) is often traded as the rarer, more popular black-bellied anglerfish (Lophius budegassa). To improve our ability to monitor and detect adulterants in these fish species, we developed a real-time PCR assay that allows for the simultaneous detection and quantification of L. piscatorius and L. budegassa. The newly designed primers target the second intron of the genomic gene parvalbumin. The proposed methodology shows good robustness, efficiency and high specificity. Of the 47 species tested, only Lophius species were amplified. Their differentiation is possible by analysing melting curves with an average Tm of 70.1 °C for L. piscatorius and 75.3 °C for L. budegassa, respectively. The detection limit for both species was 0.050 ng/μl. The assay also provides a tool to quantify parvalbumin in European anglerfish. The findings point to the use of the method proposed as a potential tool in forensic investigations to prevent illegal species substitutions, mislabeling, and food fraud.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10613 - Zoology

Projekt

<a href="/cs/project/QK1910231" target="_blank" >QK1910231: Nové přístupy k průkazu falšování rybího masa pomocí genomové DNA</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food Composition and Analysis

ISSN

0889-1575

e-ISSN

1096-0481

Svazek periodika

115

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

104992

Kód UT WoS článku

000882031000006

EID výsledku v databázi Scopus

2-s2.0-85145862660