Characterisation of the antiviral RNA interference response to Toscana virus in sand fly cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10462138" target="_blank" >RIV/00216208:11310/23:10462138 - isvavai.cz</a>
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=fp5FyzNWRO" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=fp5FyzNWRO</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.ppat.1011283" target="_blank" >10.1371/journal.ppat.1011283</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Characterisation of the antiviral RNA interference response to Toscana virus in sand fly cells
Popis výsledku v původním jazyce
Toscana virus (TOSV) (Bunyavirales, Phenuiviridae, Phlebovirus, Toscana phlebovirus) and other related human pathogenic arboviruses are transmitted by phlebotomine sand flies. TOSV has been reported in nations bordering the Mediterranean Sea among other regions. Infection can result in febrile illness as well as meningitis and encephalitis. Understanding vector-arbovirus interactions is crucial to improving our knowledge of how arboviruses spread, and in this context, immune responses that control viral replication play a significant role. Extensive research has been conducted on mosquito vector immunity against arboviruses, with RNA interference (RNAi) and specifically the exogenous siRNA (exo-siRNA) pathway playing a critical role. However, the antiviral immunity of phlebotomine sand flies is less well understood. Here we were able to show that the exo-siRNA pathway is active in a Phlebotomus papatasi-derived cell line. Following TOSV infection, distinctive 21 nucleotide virus-derived small interfering RNAs (vsiRNAs) were detected. We also identified the exo-siRNA effector Ago2 in this cell line, and silencing its expression rendered the exo-siRNA pathway largely inactive. Thus, our data show that this pathway is active as an antiviral response against a sand fly transmitted bunyavirus, TOSV. Author summaryToscana virus (TOSV) is a bunyavirus that is transmitted to humans through the bite of phlebotomine sand flies. Examining the immune responses that control arboviruses in their vectors is critical for understanding how these organisms control infection, as this may affect transmission. The exogenous siRNA (exo-siRNA) pathway is a critical antiviral immune response in mosquitoes, but much less is known about the pathway in sand flies. Here we show that a functional exo-siRNA pathway exists in a sand fly-derived cell line and that it is active as a response to TOSV infection. These findings improve our understanding of antiviral immunity in vectors in general, and phlebotomine sand flies in particular.
Název v anglickém jazyce
Characterisation of the antiviral RNA interference response to Toscana virus in sand fly cells
Popis výsledku anglicky
Toscana virus (TOSV) (Bunyavirales, Phenuiviridae, Phlebovirus, Toscana phlebovirus) and other related human pathogenic arboviruses are transmitted by phlebotomine sand flies. TOSV has been reported in nations bordering the Mediterranean Sea among other regions. Infection can result in febrile illness as well as meningitis and encephalitis. Understanding vector-arbovirus interactions is crucial to improving our knowledge of how arboviruses spread, and in this context, immune responses that control viral replication play a significant role. Extensive research has been conducted on mosquito vector immunity against arboviruses, with RNA interference (RNAi) and specifically the exogenous siRNA (exo-siRNA) pathway playing a critical role. However, the antiviral immunity of phlebotomine sand flies is less well understood. Here we were able to show that the exo-siRNA pathway is active in a Phlebotomus papatasi-derived cell line. Following TOSV infection, distinctive 21 nucleotide virus-derived small interfering RNAs (vsiRNAs) were detected. We also identified the exo-siRNA effector Ago2 in this cell line, and silencing its expression rendered the exo-siRNA pathway largely inactive. Thus, our data show that this pathway is active as an antiviral response against a sand fly transmitted bunyavirus, TOSV. Author summaryToscana virus (TOSV) is a bunyavirus that is transmitted to humans through the bite of phlebotomine sand flies. Examining the immune responses that control arboviruses in their vectors is critical for understanding how these organisms control infection, as this may affect transmission. The exogenous siRNA (exo-siRNA) pathway is a critical antiviral immune response in mosquitoes, but much less is known about the pathway in sand flies. Here we show that a functional exo-siRNA pathway exists in a sand fly-derived cell line and that it is active as a response to TOSV infection. These findings improve our understanding of antiviral immunity in vectors in general, and phlebotomine sand flies in particular.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10600 - Biological sciences
Návaznosti výsledku
Projekt
<a href="/cs/project/LX22NPO5103" target="_blank" >LX22NPO5103: Národní institut virologie a bakteriologie</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
PLoS Pathogens
ISSN
1553-7366
e-ISSN
1553-7374
Svazek periodika
19
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
18
Strana od-do
e1011283
Kód UT WoS článku
000962365700007
EID výsledku v databázi Scopus
2-s2.0-85152975641