Comparative analysis of tear cytokines in patients with glaucoma, ocular hypertension, and healthy controls

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10465958" target="_blank" >RIV/00216208:11310/23:10465958 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/23:10465958

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=yQgxSGi9dk" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=yQgxSGi9dk</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10792-023-02763-6" target="_blank" >10.1007/s10792-023-02763-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative analysis of tear cytokines in patients with glaucoma, ocular hypertension, and healthy controls

Popis výsledku v původním jazyce

Purpose:To investigate the ocular surface inflammation in patients with primary open angle glaucoma and ocular hypertension by analyzing tears and to compare findings with healthy controls.Methods:Observational case–control study. Tear samples were collected by 5 µl microcapillary tube from 24 patients with glaucoma treated by antiglaucoma drops, 9 non-treated patients with ocular hypertension and 45 healthy controls. Tears were analyzed from right eye by multiplex Bio-Plex system for the presence of 6 cytokines: IL1β, IL10, IL4, IFNγ, MIF and VEGF.Results:Significantly higher concentrations of IL1β and IL10 (glaucoma or ocular hypertension vs. healthy controls, p &lt; 0.0001), VEGF (glaucoma vs. ocular hypertension, p &lt; 0.05; ocular hypertension vs. healthy controls, p &lt; 0.02) and MIF (glaucoma vs. healthy controls, p &lt; 0.03) were detected in patients’ tears. Both patient groups have activated to a significantly lower extent the Th1 pathway represented by IFNγ than Th2 pathway represented by IL10 (p &lt; 0.001) and, at the same time, the IFNγ/IL4 ratio was significantly increased in healthy controls (p &lt; 0.001) and patients with ocular hypertension (p &lt; 0.02) compared to glaucoma individuals.Conclusion:This study shows that secretion of inflammation-related cytokines by conjunctival cells is increased in both, glaucoma and ocular hypertension patients and can be detected in their tears. Nevertheless, data indicates stronger ocular surface inflammation in non-treated follow-up patients diagnosed with ocular hypertension than in glaucoma subjects treated by antiglaucoma drops.

Název v anglickém jazyce

Comparative analysis of tear cytokines in patients with glaucoma, ocular hypertension, and healthy controls

Popis výsledku anglicky

Purpose:To investigate the ocular surface inflammation in patients with primary open angle glaucoma and ocular hypertension by analyzing tears and to compare findings with healthy controls.Methods:Observational case–control study. Tear samples were collected by 5 µl microcapillary tube from 24 patients with glaucoma treated by antiglaucoma drops, 9 non-treated patients with ocular hypertension and 45 healthy controls. Tears were analyzed from right eye by multiplex Bio-Plex system for the presence of 6 cytokines: IL1β, IL10, IL4, IFNγ, MIF and VEGF.Results:Significantly higher concentrations of IL1β and IL10 (glaucoma or ocular hypertension vs. healthy controls, p &lt; 0.0001), VEGF (glaucoma vs. ocular hypertension, p &lt; 0.05; ocular hypertension vs. healthy controls, p &lt; 0.02) and MIF (glaucoma vs. healthy controls, p &lt; 0.03) were detected in patients’ tears. Both patient groups have activated to a significantly lower extent the Th1 pathway represented by IFNγ than Th2 pathway represented by IL10 (p &lt; 0.001) and, at the same time, the IFNγ/IL4 ratio was significantly increased in healthy controls (p &lt; 0.001) and patients with ocular hypertension (p &lt; 0.02) compared to glaucoma individuals.Conclusion:This study shows that secretion of inflammation-related cytokines by conjunctival cells is increased in both, glaucoma and ocular hypertension patients and can be detected in their tears. Nevertheless, data indicates stronger ocular surface inflammation in non-treated follow-up patients diagnosed with ocular hypertension than in glaucoma subjects treated by antiglaucoma drops.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Ophthalmology

ISSN

0165-5701

e-ISSN

1573-2630

Svazek periodika

43

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

3559-3568

Kód UT WoS článku

001011184600003

EID výsledku v databázi Scopus

2-s2.0-85161886534