Transcripts of vaccinia virus postreplicative genes do not contain a 5' methylguanosine cap
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10466199" target="_blank" >RIV/00216208:11310/23:10466199 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Transcripts of vaccinia virus postreplicative genes do not contain a 5' methylguanosine cap
Popis výsledku v původním jazyce
Vaccinia virus (VACV) is a prototypical poxvirus originally used for eradication of smallpox. Investigation into VACV mRNAs carried out almost half a century agosubstantially contributed to the fundamental discovery of the 5' mRNA cap, a hallmark of all eukaryotic and many viral mRNAs. VACV research also facilitated the identification and understanding of the general mechanism of 5' mRNA capsynthesis. We characterized the VACV transcripts at the individual mRNA molecule level and found that vaccinia postreplicative mRNAs, containing nontemplated 5' poly(A) leaders, surprisingly lack the 5' cap structure in vivo. We showing that 5' cap occurrence in viral mRNAs gradually decreases in eachsuccessive gene time classes, in contrast to the reciprocal increase in 5' poly(A)leader lengths, and that these two variables are mutually negatively correlated. Wealso demonstrate that the initiator region element (INR) directly or indirectlyinuences both the frequency of 5' mRNA capping and the occurrence of 5'poly(A) leaders, including their lengths in postreplicative VACV mRNAs.
Název v anglickém jazyce
Transcripts of vaccinia virus postreplicative genes do not contain a 5' methylguanosine cap
Popis výsledku anglicky
Vaccinia virus (VACV) is a prototypical poxvirus originally used for eradication of smallpox. Investigation into VACV mRNAs carried out almost half a century agosubstantially contributed to the fundamental discovery of the 5' mRNA cap, a hallmark of all eukaryotic and many viral mRNAs. VACV research also facilitated the identification and understanding of the general mechanism of 5' mRNA capsynthesis. We characterized the VACV transcripts at the individual mRNA molecule level and found that vaccinia postreplicative mRNAs, containing nontemplated 5' poly(A) leaders, surprisingly lack the 5' cap structure in vivo. We showing that 5' cap occurrence in viral mRNAs gradually decreases in eachsuccessive gene time classes, in contrast to the reciprocal increase in 5' poly(A)leader lengths, and that these two variables are mutually negatively correlated. Wealso demonstrate that the initiator region element (INR) directly or indirectlyinuences both the frequency of 5' mRNA capping and the occurrence of 5'poly(A) leaders, including their lengths in postreplicative VACV mRNAs.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10600 - Biological sciences
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů