Specific Immune Response Against SARS-CoV-2

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10467300" target="_blank" >RIV/00216208:11310/23:10467300 - isvavai.cz</a>

Výsledek na webu

<a href="https://storage.mzevents.it/ESCV_abstract_book.pdf" target="_blank" >https://storage.mzevents.it/ESCV_abstract_book.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Specific Immune Response Against SARS-CoV-2

Popis výsledku v původním jazyce

The SARS-CoV-2 coronavirus, causing COVID-19 disease, has killed almost 7 million people worldwide. For effective treatment and introduction of appropriate antipandemic measures, it is necessary to understand the development of postinfectious and postvaccination immune responses. In our study, we analysed anti-SARS-CoV-2 immune responses mainly against the spike (S) and nucleocapsid (N) proteins and also against membrane (M) or open reading frame (O) proteins. We isolated peripheral blood mononuclear cells and serum from convalescent COVID-19 patients.Sampling was done in two rounds one year apart. Most of the patients were vaccinated in between. SARS-CoV-2 seronegative donors served as controls. We used ELISpot assays to detect SARS-CoV-2-specific cells producing IFN-γ (T cells) and IgG or IgA antibodies (B cells) using peptide mixtures (S, NMO) or recombinant proteins (S, N), respectively. We applied a CEF peptide mixture (consisting of selected epitopes from influenza A virus, cytomegalovirus, and Epstein-Barr virus) to assess the antiviral T-cell response and functionality of cells. SARS-CoV-2 specific antibodies were detected by ELISAs and a surrogate virus neutralisation assay. Our results confirmed that SARS-CoV-2 infection induces: (1) memory B cells producing IgG targeted against S and N proteins and (2) T cells producing IFN-γ after stimulation with S and NMO peptide mixtures. Interestingly, the T-cell response to the CEF mixture was lower in patients than in controls. We also detected that vaccination markedly enhanced levels of memory B cells (IgG) and T cells directed against S antigen. Comparison of B-cell responses showed a correlation between the number of IgG-producing B cells, and levels of circulating IgG and neutralizing antibodies. Our study supports the importance of vaccination for immunity boosting and suggests the possibility of long-term disruption of the antiviral immune response caused by SARS-CoV-2.

Název v anglickém jazyce

Specific Immune Response Against SARS-CoV-2

Popis výsledku anglicky

The SARS-CoV-2 coronavirus, causing COVID-19 disease, has killed almost 7 million people worldwide. For effective treatment and introduction of appropriate antipandemic measures, it is necessary to understand the development of postinfectious and postvaccination immune responses. In our study, we analysed anti-SARS-CoV-2 immune responses mainly against the spike (S) and nucleocapsid (N) proteins and also against membrane (M) or open reading frame (O) proteins. We isolated peripheral blood mononuclear cells and serum from convalescent COVID-19 patients.Sampling was done in two rounds one year apart. Most of the patients were vaccinated in between. SARS-CoV-2 seronegative donors served as controls. We used ELISpot assays to detect SARS-CoV-2-specific cells producing IFN-γ (T cells) and IgG or IgA antibodies (B cells) using peptide mixtures (S, NMO) or recombinant proteins (S, N), respectively. We applied a CEF peptide mixture (consisting of selected epitopes from influenza A virus, cytomegalovirus, and Epstein-Barr virus) to assess the antiviral T-cell response and functionality of cells. SARS-CoV-2 specific antibodies were detected by ELISAs and a surrogate virus neutralisation assay. Our results confirmed that SARS-CoV-2 infection induces: (1) memory B cells producing IgG targeted against S and N proteins and (2) T cells producing IFN-γ after stimulation with S and NMO peptide mixtures. Interestingly, the T-cell response to the CEF mixture was lower in patients than in controls. We also detected that vaccination markedly enhanced levels of memory B cells (IgG) and T cells directed against S antigen. Comparison of B-cell responses showed a correlation between the number of IgG-producing B cells, and levels of circulating IgG and neutralizing antibodies. Our study supports the importance of vaccination for immunity boosting and suggests the possibility of long-term disruption of the antiviral immune response caused by SARS-CoV-2.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30102 - Immunology

Projekt

<a href="/cs/project/LX22NPO5103" target="_blank" >LX22NPO5103: Národní institut virologie a bakteriologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů