Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10472887" target="_blank" >RIV/00216208:11310/23:10472887 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=.rjSPmwvyt" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=.rjSPmwvyt</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/s23073425" target="_blank" >10.3390/s23073425</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor

Popis výsledku v původním jazyce

Engineered nanomaterials are becoming increasingly common in commercial and consumer products and pose a serious toxicological threat. Exposure of human organisms to nanomaterials can occur by inhalation, oral intake, or dermal transport. Together with the consumption of alcohol in the physiological environment of the body containing NaCl, this has raised concerns about the potentially harmful effects of ingested nanomaterials on human health. Although gold nanoparticles (AuNPs) exhibit great potential for various biomedical applications, there is some inconsistency in the case of the unambiguous genotoxicity of AuNPs due to differences in their shape, size, solubility, and exposure time. A DNA/GCE (DNA/glassy carbon electrode) biosensor was used to study ethanol (EtOH) and NaCl-induced gold nanoparticle aggregation genotoxicity under UV light in this study. The genotoxic effect of dispersed and aggregated negatively charged gold nanoparticles AuNP1 (8 nm) and AuNP2 (30 nm) toward salmon sperm double-stranded dsDNA was monitored by cyclic and square-wave voltammetry (CV, SWV). Electrochemical impedance spectroscopy (EIS) was used for a surface study of the biosensor. The aggregation of AuNPs was monitored by UV-vis spectroscopy. AuNP1 aggregates formed by 30% v/v EtOH and 0.15 mol.L(-1) NaCl caused the greatest damage to the biosensor DNA layer.

Název v anglickém jazyce

Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor

Popis výsledku anglicky

Engineered nanomaterials are becoming increasingly common in commercial and consumer products and pose a serious toxicological threat. Exposure of human organisms to nanomaterials can occur by inhalation, oral intake, or dermal transport. Together with the consumption of alcohol in the physiological environment of the body containing NaCl, this has raised concerns about the potentially harmful effects of ingested nanomaterials on human health. Although gold nanoparticles (AuNPs) exhibit great potential for various biomedical applications, there is some inconsistency in the case of the unambiguous genotoxicity of AuNPs due to differences in their shape, size, solubility, and exposure time. A DNA/GCE (DNA/glassy carbon electrode) biosensor was used to study ethanol (EtOH) and NaCl-induced gold nanoparticle aggregation genotoxicity under UV light in this study. The genotoxic effect of dispersed and aggregated negatively charged gold nanoparticles AuNP1 (8 nm) and AuNP2 (30 nm) toward salmon sperm double-stranded dsDNA was monitored by cyclic and square-wave voltammetry (CV, SWV). Electrochemical impedance spectroscopy (EIS) was used for a surface study of the biosensor. The aggregation of AuNPs was monitored by UV-vis spectroscopy. AuNP1 aggregates formed by 30% v/v EtOH and 0.15 mol.L(-1) NaCl caused the greatest damage to the biosensor DNA layer.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors

ISSN

1424-8220

e-ISSN

1424-8220

Svazek periodika

23

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

3425

Kód UT WoS článku

000970283800001

EID výsledku v databázi Scopus

2-s2.0-85152346985