Toxin rescue by a random sequence : A random sequence variant in an experimental screen can rescue Escherichi coli from the deleterious effects of a RNase toxin by interacting with chaperones

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10477001" target="_blank" >RIV/00216208:11310/23:10477001 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=04Lx9J4kMF" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=04Lx9J4kMF</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41559-023-02252-0" target="_blank" >10.1038/s41559-023-02252-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Toxin rescue by a random sequence : A random sequence variant in an experimental screen can rescue Escherichi coli from the deleterious effects of a RNase toxin by interacting with chaperones

Popis výsledku v původním jazyce

Many sequences identified through transcriptomic and proteomic technologies do not align with annotated genes1 . Across various species, hundreds to thousands of noncanonical open reading frames (ORFs) have been detected, and many of these are associated with detectable levels of protein (for example, ref. 2). These ORFs are typically much shorter than well-characterized and evolutionary conserved genes and are present in substantially lower quantities3 . Most such sequences are rapidly purged by selection, but numerous instances of surviving proteins originating de novo from previously noncoding DNA have been documented4 . Although the mechanisms that govern their emergence and adaptation remain poorly understood, these sequences represent ongoing evolutionary &apos;experiments&apos;. This raises the question of how frequently random sequences can interact with the cellular environment in non-deleterious ways, and how often they can assume novel functional roles. Writing in this issue of Nature Ecology &amp; Evolution, Frumkin and Laub5 tackle these questions by searching sequence space for random sequences that rescue E. coli from the deleterious effects of the RNase toxin MazF.

Název v anglickém jazyce

Toxin rescue by a random sequence : A random sequence variant in an experimental screen can rescue Escherichi coli from the deleterious effects of a RNase toxin by interacting with chaperones

Popis výsledku anglicky

Many sequences identified through transcriptomic and proteomic technologies do not align with annotated genes1 . Across various species, hundreds to thousands of noncanonical open reading frames (ORFs) have been detected, and many of these are associated with detectable levels of protein (for example, ref. 2). These ORFs are typically much shorter than well-characterized and evolutionary conserved genes and are present in substantially lower quantities3 . Most such sequences are rapidly purged by selection, but numerous instances of surviving proteins originating de novo from previously noncoding DNA have been documented4 . Although the mechanisms that govern their emergence and adaptation remain poorly understood, these sequences represent ongoing evolutionary &apos;experiments&apos;. This raises the question of how frequently random sequences can interact with the cellular environment in non-deleterious ways, and how often they can assume novel functional roles. Writing in this issue of Nature Ecology &amp; Evolution, Frumkin and Laub5 tackle these questions by searching sequence space for random sequences that rescue E. coli from the deleterious effects of the RNase toxin MazF.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

NATURE ECOLOGY &amp; EVOLUTION

ISSN

2397-334X

e-ISSN

2397-334X

Svazek periodika

7

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

2

Strana od-do

1963-1964

Kód UT WoS článku

001157067100001

EID výsledku v databázi Scopus

2-s2.0-85176565097