High-throughput optical imaging technology for large-scale single-cell analysis of live Euglena gracilis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F24%3A10485792" target="_blank" >RIV/00216208:11310/24:10485792 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=s1RiPPHZSG" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=s1RiPPHZSG</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.trac.2024.117938" target="_blank" >10.1016/j.trac.2024.117938</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High-throughput optical imaging technology for large-scale single-cell analysis of live Euglena gracilis

Popis výsledku v původním jazyce

Euglena gracilis, a flagellated, single-celled organism shaped like a spindle, belongs to the Euglenozoa phylum. This protist is predominantly found in freshwater bodies such as lakes and ponds. Unique in its ability to perform photosynthesis and heterotrophic feeding, E. gracilis can produce organic materials on its own or absorb them from the environment. This intriguing blend of plant and animal traits, coupled with promising biotechnological applications, have made it a subject of extensive research. Notably, E. gracilis has been used for biofuel production and synthesis of various compounds such as amino acids and vitamins. For these applications, rapid assessment of single E. gracilis cells within populations is essential. This paper introduces recent advancements in high-throughput optical imaging technology that enables large-scale, single-cell analysis of E. gracilis. We review the benefits and limitations of various methods within this technology, along with discussing its future potential.

Název v anglickém jazyce

High-throughput optical imaging technology for large-scale single-cell analysis of live Euglena gracilis

Popis výsledku anglicky

Euglena gracilis, a flagellated, single-celled organism shaped like a spindle, belongs to the Euglenozoa phylum. This protist is predominantly found in freshwater bodies such as lakes and ponds. Unique in its ability to perform photosynthesis and heterotrophic feeding, E. gracilis can produce organic materials on its own or absorb them from the environment. This intriguing blend of plant and animal traits, coupled with promising biotechnological applications, have made it a subject of extensive research. Notably, E. gracilis has been used for biofuel production and synthesis of various compounds such as amino acids and vitamins. For these applications, rapid assessment of single E. gracilis cells within populations is essential. This paper introduces recent advancements in high-throughput optical imaging technology that enables large-scale, single-cell analysis of E. gracilis. We review the benefits and limitations of various methods within this technology, along with discussing its future potential.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Trends in Analytical Chemistry

ISSN

0165-9936

e-ISSN

1879-3142

Svazek periodika

180

Číslo periodika v rámci svazku

November

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

117938

Kód UT WoS článku

001308190100001

EID výsledku v databázi Scopus

2-s2.0-85202851681