Integrating vMAGs and Amplicon Sequencing: Bridging Gaps in Illumina-Based Assembly of Novel Large Viruses from Honey Bee Viromes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F24%3A10488307" target="_blank" >RIV/00216208:11310/24:10488307 - isvavai.cz</a>
Výsledek na webu
<a href="https://evbc.uni-jena.de/events/vibiom2024/" target="_blank" >https://evbc.uni-jena.de/events/vibiom2024/</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Integrating vMAGs and Amplicon Sequencing: Bridging Gaps in Illumina-Based Assembly of Novel Large Viruses from Honey Bee Viromes
Popis výsledku v původním jazyce
The study of viromes has been revolutionized by advanced bioinformatics methodologies that enable the precise identification of novel viruses. However, assembling complete genomes of large viruses is still challenging. To address these issues, we employed a viral binning approach that integrates fragmented sequences to reconstruct viral genomes. Our methodology focused on identifying and elongating contigs and reducing their number where feasible. Additionally, we complemented our efforts with wet laboratory techniques, specifically generatingandsequencing PCR products, to ensure genome completeness. Our methodological approach revealed complete genomes of previously unidentified large DNA viruses in the honey bee population.
Název v anglickém jazyce
Integrating vMAGs and Amplicon Sequencing: Bridging Gaps in Illumina-Based Assembly of Novel Large Viruses from Honey Bee Viromes
Popis výsledku anglicky
The study of viromes has been revolutionized by advanced bioinformatics methodologies that enable the precise identification of novel viruses. However, assembling complete genomes of large viruses is still challenging. To address these issues, we employed a viral binning approach that integrates fragmented sequences to reconstruct viral genomes. Our methodology focused on identifying and elongating contigs and reducing their number where feasible. Additionally, we complemented our efforts with wet laboratory techniques, specifically generatingandsequencing PCR products, to ensure genome completeness. Our methodological approach revealed complete genomes of previously unidentified large DNA viruses in the honey bee population.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10607 - Virology
Návaznosti výsledku
Projekt
<a href="/cs/project/LX22NPO5103" target="_blank" >LX22NPO5103: Národní institut virologie a bakteriologie</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2024
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů