Asaia bacteria in sand flies and their impact on Leishmania transmission

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F24%3A10489551" target="_blank" >RIV/00216208:11310/24:10489551 - isvavai.cz</a>

Výsledek na webu

<a href="http://isops11.si/wp-content/uploads/2024/12/ISOPSXI-Abstract-Book-PDF-2.12.2024.pdf" target="_blank" >http://isops11.si/wp-content/uploads/2024/12/ISOPSXI-Abstract-Book-PDF-2.12.2024.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Asaia bacteria in sand flies and their impact on Leishmania transmission

Popis výsledku v původním jazyce

Midgut microbiome was demonstrated to affect the transmission of vector-borne pathogens. In sand flies, Asaia bacteria was found as a part diet and midgut microbiome. Here, we have investigated the effect of two Asaia species on the development of Leishmania major in Phlebotomus duboscqi. The sand flies were first infected with bacteria via sugar meal and then membrane-fed on blood containing Leishmania promasgotes. Following this superinfecon, the development of Leishmania infecon was examined. Parcularly, we studied changes in localizaon and intensity of infecon and examined Leishmania morphological forms on midgut smears. Both tested bacteria species, Asaia siamensis, and Asaia krungthepensis, colonized the intesne of female Ph. Duboscqi for up to 8 days aer infecon and were transmied vercally to the next generaon through contaminaon of the egg surface. The presence of Asaia within Ph. Duboscqi negavely affects the intensity of Leishmania late-stage infecons. In addion to the wild type, we tested a strain of Asaia engineered for the expression of a protein of Wolbachia (WSP). This strain of Asaia also readily survives in Ph. duboscqi midgut and experiments on its effect on Leishmania infecon are in progress.

Název v anglickém jazyce

Asaia bacteria in sand flies and their impact on Leishmania transmission

Popis výsledku anglicky

Midgut microbiome was demonstrated to affect the transmission of vector-borne pathogens. In sand flies, Asaia bacteria was found as a part diet and midgut microbiome. Here, we have investigated the effect of two Asaia species on the development of Leishmania major in Phlebotomus duboscqi. The sand flies were first infected with bacteria via sugar meal and then membrane-fed on blood containing Leishmania promasgotes. Following this superinfecon, the development of Leishmania infecon was examined. Parcularly, we studied changes in localizaon and intensity of infecon and examined Leishmania morphological forms on midgut smears. Both tested bacteria species, Asaia siamensis, and Asaia krungthepensis, colonized the intesne of female Ph. Duboscqi for up to 8 days aer infecon and were transmied vercally to the next generaon through contaminaon of the egg surface. The presence of Asaia within Ph. Duboscqi negavely affects the intensity of Leishmania late-stage infecons. In addion to the wild type, we tested a strain of Asaia engineered for the expression of a protein of Wolbachia (WSP). This strain of Asaia also readily survives in Ph. duboscqi midgut and experiments on its effect on Leishmania infecon are in progress.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10600 - Biological sciences

Projekt

<a href="/cs/project/LX22NPO5103" target="_blank" >LX22NPO5103: Národní institut virologie a bakteriologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů