Diversity and Multiple Infections of Bartonella in Red Deer and Deer Keds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F25%3A10493262" target="_blank" >RIV/00216208:11310/25:10493262 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/75010330:_____/25:00014988

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=iJipT7aGPh" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=iJipT7aGPh</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/pathogens14010006" target="_blank" >10.3390/pathogens14010006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Diversity and Multiple Infections of Bartonella in Red Deer and Deer Keds

Popis výsledku v původním jazyce

Bartonellae are zoonotic pathogens with a broad range of reservoir hosts and vectors. To examine sylvatic Bartonella reservoirs, tissue samples of red deer (Cervus elaphus, n = 114) and their associated deer keds (Lipoptena cervi, n = 50; L. fortisetosa, n = 272) collected in the Czech Republic were tested for the presence of Bartonella using PCR at four loci (gltA, rpoB, nuoG, ITS); PCR sensitivity was increased significantly by using primers modified for the detection of wildlife-associated bartonellae. One-third of the deer and 70% of the deer keds were Bartonella positive; within the tested animal tissues, usually the spleen was positive. The most prevalent Bartonella represents an undescribed species related to isolates from Japanese sika deer and L. fortisetosa. Additionally, B. schoenbuchensis sensu lato and B. bovis were found, together making up 17 genotypes characterized by multi-locus sequence typing, all unique compared to previously published sequences. Nanopore sequencing of selected samples revealed an additional 14 unique Bartonella genotypes, with up to six genotypes co-infecting one deer, highlighting the diversity of ruminant Bartonella. The high COI variety of examined L. cervi and L. fortisetosa suggests L. fortisetosa in central Europe is not a homogenous invasive population.

Název v anglickém jazyce

Diversity and Multiple Infections of Bartonella in Red Deer and Deer Keds

Popis výsledku anglicky

Bartonellae are zoonotic pathogens with a broad range of reservoir hosts and vectors. To examine sylvatic Bartonella reservoirs, tissue samples of red deer (Cervus elaphus, n = 114) and their associated deer keds (Lipoptena cervi, n = 50; L. fortisetosa, n = 272) collected in the Czech Republic were tested for the presence of Bartonella using PCR at four loci (gltA, rpoB, nuoG, ITS); PCR sensitivity was increased significantly by using primers modified for the detection of wildlife-associated bartonellae. One-third of the deer and 70% of the deer keds were Bartonella positive; within the tested animal tissues, usually the spleen was positive. The most prevalent Bartonella represents an undescribed species related to isolates from Japanese sika deer and L. fortisetosa. Additionally, B. schoenbuchensis sensu lato and B. bovis were found, together making up 17 genotypes characterized by multi-locus sequence typing, all unique compared to previously published sequences. Nanopore sequencing of selected samples revealed an additional 14 unique Bartonella genotypes, with up to six genotypes co-infecting one deer, highlighting the diversity of ruminant Bartonella. The high COI variety of examined L. cervi and L. fortisetosa suggests L. fortisetosa in central Europe is not a homogenous invasive population.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NU23-05-00511" target="_blank" >NU23-05-00511: Bartonelóza v ČR jako přehlížené onemocnění: zdroje a rizika infekcí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pathogens

ISSN

2076-0817

e-ISSN

2076-0817

Svazek periodika

14

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

14

Strana od-do

6

Kód UT WoS článku

001403869900001

EID výsledku v databázi Scopus

2-s2.0-85215792967