A simplified system for the detection of antennal responses to host-borne volatile organic compounds in sand flies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F25%3A10501333" target="_blank" >RIV/00216208:11310/25:10501333 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=tOww-2ZM13" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=tOww-2ZM13</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13071-025-06998-3" target="_blank" >10.1186/s13071-025-06998-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A simplified system for the detection of antennal responses to host-borne volatile organic compounds in sand flies

Popis výsledku v původním jazyce

Phlebotomus (Larroussius) perniciosus (Diptera: Psychodidae) is the most common and predominant vector of Leishmania infantum in the Western Mediterranean region. Volatile organic compounds (VOCs) produced by vertebrates are important cues affecting the behaviour of blood-feeding insects. Generally, the identification of putative behaviourally active VOCs involves three distinct phases: extraction, chemical characterization and chemoreceptivity evaluation using electrophysiological techniques. Here, we present a simplified gas chromatography-mass spectrometry-electroantennographic detection (GC-MS-EAD) setup adapted for screening bioactive compounds in sand flies, in which the chemical identification and antennal responses are recorded simultaneously.The method integrates: (i) a flow-splitter that balances the flow rate of the two outgoing streams, (ii) GC columns with different lengths and diameters in the two sections splitter-MS and splitter-EAD and (iii) an antennal signal amplifier. The GC-MS-EAD analysis was applied to headspace solid-phase microextraction (HS-SPME) extracts from a healthy dog, and antennal responses were recorded in female P. perniciosus sand flies.The canine VOC profile was predominantly composed of aldehydes, with hexanal and nonanal eliciting the strongest antennal responses in P. perniciosus.This simplified GC-MS-EAD system shows promise for broader application in the study of host-vector interactions. Its use across different host-vector pairs may enhance our understanding of these relationships and inform the development of strategies for integrated vector monitoring and control.

Název v anglickém jazyce

A simplified system for the detection of antennal responses to host-borne volatile organic compounds in sand flies

Popis výsledku anglicky

Phlebotomus (Larroussius) perniciosus (Diptera: Psychodidae) is the most common and predominant vector of Leishmania infantum in the Western Mediterranean region. Volatile organic compounds (VOCs) produced by vertebrates are important cues affecting the behaviour of blood-feeding insects. Generally, the identification of putative behaviourally active VOCs involves three distinct phases: extraction, chemical characterization and chemoreceptivity evaluation using electrophysiological techniques. Here, we present a simplified gas chromatography-mass spectrometry-electroantennographic detection (GC-MS-EAD) setup adapted for screening bioactive compounds in sand flies, in which the chemical identification and antennal responses are recorded simultaneously.The method integrates: (i) a flow-splitter that balances the flow rate of the two outgoing streams, (ii) GC columns with different lengths and diameters in the two sections splitter-MS and splitter-EAD and (iii) an antennal signal amplifier. The GC-MS-EAD analysis was applied to headspace solid-phase microextraction (HS-SPME) extracts from a healthy dog, and antennal responses were recorded in female P. perniciosus sand flies.The canine VOC profile was predominantly composed of aldehydes, with hexanal and nonanal eliciting the strongest antennal responses in P. perniciosus.This simplified GC-MS-EAD system shows promise for broader application in the study of host-vector interactions. Its use across different host-vector pairs may enhance our understanding of these relationships and inform the development of strategies for integrated vector monitoring and control.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasites &amp; Vectors

ISSN

1756-3305

e-ISSN

1756-3305

Svazek periodika

18

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

352

Kód UT WoS článku

001554048200002

EID výsledku v databázi Scopus

2-s2.0-105013582545