Seeing in the Deep: Evolution of the Opsin Gene Expression in Bermin Crater Lake Cichlids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F25%3A10505852" target="_blank" >RIV/00216208:11310/25:10505852 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=16VGhw26mG" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=16VGhw26mG</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/molbev/msaf286" target="_blank" >10.1093/molbev/msaf286</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Seeing in the Deep: Evolution of the Opsin Gene Expression in Bermin Crater Lake Cichlids

Popis výsledku v původním jazyce

Cichlid visual systems can evolve rapidly during adaptive radiations. The Bermin crater lake species flock in Cameroon, comprising 13 (nine valid and four undescribed) Coptodon species, offers an ideal model to investigate visual adaptation to the deep-water light environments. Here, we examine visual opsin genes sequences and expression using 109 retina transcriptomes, focusing on interspecific variation with habitat depth, as well as on seasonal changes in the migratory species between depths. All species possess a multichromatic system with at least five cone opsins. While opsin coding sequences show limited divergence - consistent with the flock&apos;s recent origin - opsin expression profiles vary substantially. Deep-water species showed reduced sws1 and sws2b expression, in line with lower UV and violet light availability in deeper waters. Unexpectedly, proportional expression of the red-sensitive lws opsin gene increases with depth, contrasting with patterns in other lacustrine cichlids. In the seasonally migrating species Coptodon imbrifernus, opsin expression is plastic, with decreased sws2b levels in deeper-dwelling dry-season individuals. To contextualize our findings, we compared Bermin cichlids to the older Barombi Mbo crater lake radiation. While single cone adaptations to the depth were convergent (loss of UV/violet sensitivity, enhanced blue sensitivity), double cone response diverged: lws expression was lost in Barombi Mbo while increased in Bermin deep-water species. Our findings suggest that plasticity in opsin expression plays a crucial role at the onset of sensory evolution, potentially paving the way for future genetic change. This study underscores the power of young systems like Bermin for uncovering the mechanisms driving early visual system diversification.

Název v anglickém jazyce

Seeing in the Deep: Evolution of the Opsin Gene Expression in Bermin Crater Lake Cichlids

Popis výsledku anglicky

Cichlid visual systems can evolve rapidly during adaptive radiations. The Bermin crater lake species flock in Cameroon, comprising 13 (nine valid and four undescribed) Coptodon species, offers an ideal model to investigate visual adaptation to the deep-water light environments. Here, we examine visual opsin genes sequences and expression using 109 retina transcriptomes, focusing on interspecific variation with habitat depth, as well as on seasonal changes in the migratory species between depths. All species possess a multichromatic system with at least five cone opsins. While opsin coding sequences show limited divergence - consistent with the flock&apos;s recent origin - opsin expression profiles vary substantially. Deep-water species showed reduced sws1 and sws2b expression, in line with lower UV and violet light availability in deeper waters. Unexpectedly, proportional expression of the red-sensitive lws opsin gene increases with depth, contrasting with patterns in other lacustrine cichlids. In the seasonally migrating species Coptodon imbrifernus, opsin expression is plastic, with decreased sws2b levels in deeper-dwelling dry-season individuals. To contextualize our findings, we compared Bermin cichlids to the older Barombi Mbo crater lake radiation. While single cone adaptations to the depth were convergent (loss of UV/violet sensitivity, enhanced blue sensitivity), double cone response diverged: lws expression was lost in Barombi Mbo while increased in Bermin deep-water species. Our findings suggest that plasticity in opsin expression plays a crucial role at the onset of sensory evolution, potentially paving the way for future genetic change. This study underscores the power of young systems like Bermin for uncovering the mechanisms driving early visual system diversification.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

<a href="/cs/project/GJ16-09784Y" target="_blank" >GJ16-09784Y: Adaptace na život v hloubce: evoluce opsinových a hemogobinových genů na modelu cichlid</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Biology and Evolution

ISSN

0737-4038

e-ISSN

1537-1719

Svazek periodika

42

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

18

Strana od-do

msaf286

Kód UT WoS článku

001620604900001

EID výsledku v databázi Scopus

2-s2.0-105022705285