Substrate-Independent Approach for the Generation of Functional Protein Resistant Surfaces

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F11%3A10104989" target="_blank" >RIV/00216208:11320/11:10104989 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/11:00359207

Výsledek na webu

<a href="http://pubs.acs.org/doi/abs/10.1021/bm101406m" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/bm101406m</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/bm101406m" target="_blank" >10.1021/bm101406m</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Substrate-Independent Approach for the Generation of Functional Protein Resistant Surfaces

Popis výsledku v původním jazyce

A new route for coating various substrates with antifouling polymer layers was developed. Polymer brushes of hydroxy- and methoxy-capped oligoethyleneglycol methacrylate and carboxybetaine acrylamide were grafted from bromoisobutyrate initiator attachedto a 15 nm thick amino-rich adhesion layer deposited on diverse substrates by RF magnetron sputtering of Nylon 6,6. Well-controlled polymerization kinetics made it possible to control the thickness of the brushes at a nanometer scale. Zero fouling from single protein solutions and a reduction of more than 90% in the fouling from blood plasma observed oh the uncoated surfaces was achieved. The feasibility of functionalization with bioactive compounds was tested by covalent attachment of streptavidin ontopoly(oligoethylene glycol methacrylate) brush and subsequent immobilization of model antibodies and "oligonudecitides.

Název v anglickém jazyce

Substrate-Independent Approach for the Generation of Functional Protein Resistant Surfaces

Popis výsledku anglicky

A new route for coating various substrates with antifouling polymer layers was developed. Polymer brushes of hydroxy- and methoxy-capped oligoethyleneglycol methacrylate and carboxybetaine acrylamide were grafted from bromoisobutyrate initiator attachedto a 15 nm thick amino-rich adhesion layer deposited on diverse substrates by RF magnetron sputtering of Nylon 6,6. Well-controlled polymerization kinetics made it possible to control the thickness of the brushes at a nanometer scale. Zero fouling from single protein solutions and a reduction of more than 90% in the fouling from blood plasma observed oh the uncoated surfaces was achieved. The feasibility of functionalization with bioactive compounds was tested by covalent attachment of streptavidin ontopoly(oligoethylene glycol methacrylate) brush and subsequent immobilization of model antibodies and "oligonudecitides.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BM - Fyzika pevných látek a magnetismus

OECD FORD obor

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Projekt

<a href="/cs/project/KAN200670701" target="_blank" >KAN200670701: Biosenzory s povrchovými plasmony a proteinové čipy pro lékařskou diagnostiku</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomacromolecules

ISSN

1525-7797

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1058-1066

Kód UT WoS článku

000289223500024

EID výsledku v databázi Scopus

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