Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F13%3A10143976" target="_blank" >RIV/00216208:11320/13:10143976 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/13:00392660 RIV/00216224:14740/13:00067549

Výsledek na webu

<a href="http://dx.doi.org/10.1093/nar/gks1135" target="_blank" >http://dx.doi.org/10.1093/nar/gks1135</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gks1135" target="_blank" >10.1093/nar/gks1135</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study

Popis výsledku v původním jazyce

DNA concentration has been recently suggested to be the reason why different arrangements are revealed for K+-stabilized human telomere quadruplexes by experimental methods requiring DNA concentrations differing by orders of magnitude. As Raman spectroscopy can be applied to DNA samples ranging from those accessible by absorption and CD spectroscopies up to extremely concentrated solutions, gels and even crystals; it has been used here to clarify polymorphism of a core human telomeric sequence G(3)(TTAG(3))(3) in the presence of K+ and Na+ ions throughout wide range of DNA concentrations. We demonstrate that the K+-structure of G(3)(TTAG(3))(3) at low DNA concentration is close to the antiparallel fold of Na+-stabilized quadruplex. On the increase of G(3)(TTAG(3))(3) concentration, a gradual transition from antiparallel to intramolecular parallel arrangement was observed, but only for thermodynamically equilibrated K+-stabilized samples. The transition is synergically supported by incr

Název v anglickém jazyce

Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study

Popis výsledku anglicky

DNA concentration has been recently suggested to be the reason why different arrangements are revealed for K+-stabilized human telomere quadruplexes by experimental methods requiring DNA concentrations differing by orders of magnitude. As Raman spectroscopy can be applied to DNA samples ranging from those accessible by absorption and CD spectroscopies up to extremely concentrated solutions, gels and even crystals; it has been used here to clarify polymorphism of a core human telomeric sequence G(3)(TTAG(3))(3) in the presence of K+ and Na+ ions throughout wide range of DNA concentrations. We demonstrate that the K+-structure of G(3)(TTAG(3))(3) at low DNA concentration is close to the antiparallel fold of Na+-stabilized quadruplex. On the increase of G(3)(TTAG(3))(3) concentration, a gradual transition from antiparallel to intramolecular parallel arrangement was observed, but only for thermodynamically equilibrated K+-stabilized samples. The transition is synergically supported by incr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Svazek periodika

41

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

1005-1016

Kód UT WoS článku

000314121100034

EID výsledku v databázi Scopus

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