Distinguishing co-existing unimolecular and bimolecular structural forms using joint analysis of Raman and UV absorption data

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F15%3A10314373" target="_blank" >RIV/00216208:11320/15:10314373 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jrs.4610" target="_blank" >http://dx.doi.org/10.1002/jrs.4610</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jrs.4610" target="_blank" >10.1002/jrs.4610</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Distinguishing co-existing unimolecular and bimolecular structural forms using joint analysis of Raman and UV absorption data

Popis výsledku v původním jazyce

Standard Raman measurements of solutions require relatively high concentration of dissolved species, which supports formation of intermolecular complexes. In case of simultaneous presence of unimolecular and bimolecular structural forms, it is often difficult or even impossible to discriminate between them by using only the Raman data. We present a possible and effective way how to combine temperature dependencies of Raman scattering and UV absorption in a joint thermodynamic analysis. This approach isbased on a joint fit of the results of multivariate analysis (singular value decomposition) of individual Raman and UV absorption spectral sets to a thermodynamic model. Our case study on RNA oligonucleotides, which can form imperfect intermolecular duplexes or intramolecular hairpin structures, demonstrates that this approach allows to determine thermodynamic parameters of co-existing unimolecular and bimolecular structural forms and to isolate their Raman spectra. Sons, Ltd.

Název v anglickém jazyce

Distinguishing co-existing unimolecular and bimolecular structural forms using joint analysis of Raman and UV absorption data

Popis výsledku anglicky

Standard Raman measurements of solutions require relatively high concentration of dissolved species, which supports formation of intermolecular complexes. In case of simultaneous presence of unimolecular and bimolecular structural forms, it is often difficult or even impossible to discriminate between them by using only the Raman data. We present a possible and effective way how to combine temperature dependencies of Raman scattering and UV absorption in a joint thermodynamic analysis. This approach isbased on a joint fit of the results of multivariate analysis (singular value decomposition) of individual Raman and UV absorption spectral sets to a thermodynamic model. Our case study on RNA oligonucleotides, which can form imperfect intermolecular duplexes or intramolecular hairpin structures, demonstrates that this approach allows to determine thermodynamic parameters of co-existing unimolecular and bimolecular structural forms and to isolate their Raman spectra. Sons, Ltd.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

<a href="/cs/project/GA202%2F09%2F0193" target="_blank" >GA202/09/0193: Vznik a dynamika strukturních motivů nukleových kyselin podílejících se na regulaci genové exprese</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Raman Spectroscopy

ISSN

0377-0486

e-ISSN

—

Svazek periodika

46

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

244-250

Kód UT WoS článku

000349891900007

EID výsledku v databázi Scopus

2-s2.0-84923040243