Drop-Coating Deposition Raman (DCDR) Spectroscopy as a Tool for Membrane Interaction Studies: Liposome Porphyrin Complex

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F15%3A10314878" target="_blank" >RIV/00216208:11320/15:10314878 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1366/14-07836" target="_blank" >http://dx.doi.org/10.1366/14-07836</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1366/14-07836" target="_blank" >10.1366/14-07836</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Drop-Coating Deposition Raman (DCDR) Spectroscopy as a Tool for Membrane Interaction Studies: Liposome Porphyrin Complex

Popis výsledku v původním jazyce

Drop-coating deposition Raman (DCDR) spectroscopy is based on the measurement of a sample that has been preconcentrated by being dried on a special hydrophobic plate. In addition to its higher sensitivity, the advantage of DCDR over the conventional Raman spectroscopy is the small sample volume needed, the lack of interference from solvents, and the capability of segregating any impurities present and separating components in more complex samples. In this study, DCDR spectroscopy was employed to investigate the complex of the cationic copper(II) 5,10,15,20-tetrakis(1-methyl-4-pyridyl) porphyrin (CuTMPyP) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes. Drop-coating deposition Raman spectra were treated using factor analysis (FA), whichled to the following conclusions: (i) the distribution of CuTMPyP in the complex is not homogenous, (ii) the DCDR technique segregates complexed and noncomplexed parts of the sample, (iii) the spectral changes caused by the drying proces

Název v anglickém jazyce

Drop-Coating Deposition Raman (DCDR) Spectroscopy as a Tool for Membrane Interaction Studies: Liposome Porphyrin Complex

Popis výsledku anglicky

Drop-coating deposition Raman (DCDR) spectroscopy is based on the measurement of a sample that has been preconcentrated by being dried on a special hydrophobic plate. In addition to its higher sensitivity, the advantage of DCDR over the conventional Raman spectroscopy is the small sample volume needed, the lack of interference from solvents, and the capability of segregating any impurities present and separating components in more complex samples. In this study, DCDR spectroscopy was employed to investigate the complex of the cationic copper(II) 5,10,15,20-tetrakis(1-methyl-4-pyridyl) porphyrin (CuTMPyP) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes. Drop-coating deposition Raman spectra were treated using factor analysis (FA), whichled to the following conclusions: (i) the distribution of CuTMPyP in the complex is not homogenous, (ii) the DCDR technique segregates complexed and noncomplexed parts of the sample, (iii) the spectral changes caused by the drying proces

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

<a href="/cs/project/GBP205%2F12%2FG118" target="_blank" >GBP205/12/G118: Nanobiofotonika pro medicínu budoucnosti</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Spectroscopy

ISSN

0003-7028

e-ISSN

—

Svazek periodika

69

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

939-945

Kód UT WoS článku

000359327600005

EID výsledku v databázi Scopus

2-s2.0-84956573916