Gold film over very small (107nm) spheres as efficient substrate for sensitive and reproducible surface-enhanced Raman scattering (SERS) detection of biologically important molecules

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F18%3A10386423" target="_blank" >RIV/00216208:11320/18:10386423 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21340/18:00319912

Výsledek na webu

<a href="https://doi.org/10.1002/jrs.5317" target="_blank" >https://doi.org/10.1002/jrs.5317</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jrs.5317" target="_blank" >10.1002/jrs.5317</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gold film over very small (107nm) spheres as efficient substrate for sensitive and reproducible surface-enhanced Raman scattering (SERS) detection of biologically important molecules

Popis výsledku v původním jazyce

Gold plasmonic nanostructures with high sensitivity and spectral reproducibility are key components of molecular sensors based on surface-enhanced Raman scattering (SERS). In this paper, we report a bottom-up fabrication of gold film over nanosphere (FON) substrates for SERS sensing on the basis of very small (107nm in diameter) polystyrene spheres coated with 20nm of gold. To obtain close-packed spheres in a large scale area, the self-assembly at the water-air interface was found to be very efficient. Sensitivity and reproducibility of the AuFON substrates were tested using various molecular probes: 5,10,15,20-tetrakis(1-methyl-4-pyridyl)porphyrin, p-aminothiophenol, and benzocaine. Relative standard deviation of SERS signal was found to be less than 20% confirming good spectral reproducibility especially using a 100x objective. Then, we demonstrated sensitive SERS detection of other biologically important molecules adsorbed on our AuFON substrates: thiolated-polyA, protoporphyrin IX, and two alkaloids (nicotine and strychnine). Estimated limits of detection were 8.2x10(-7) and 4.5x10(-7)M (in order of 100ng.ml(-1)) incubation concentration for strychnine and nicotine, respectively. Therefore, we conclude that the 20-nm gold film deposited on polystyrene spheres of 107nm in diameter provided both good spectral reproducibility and sensitivity. Their implementation into the SERS-active system will enable to bring about new quantitative and analytical SERS applications.

Název v anglickém jazyce

Gold film over very small (107nm) spheres as efficient substrate for sensitive and reproducible surface-enhanced Raman scattering (SERS) detection of biologically important molecules

Popis výsledku anglicky

Gold plasmonic nanostructures with high sensitivity and spectral reproducibility are key components of molecular sensors based on surface-enhanced Raman scattering (SERS). In this paper, we report a bottom-up fabrication of gold film over nanosphere (FON) substrates for SERS sensing on the basis of very small (107nm in diameter) polystyrene spheres coated with 20nm of gold. To obtain close-packed spheres in a large scale area, the self-assembly at the water-air interface was found to be very efficient. Sensitivity and reproducibility of the AuFON substrates were tested using various molecular probes: 5,10,15,20-tetrakis(1-methyl-4-pyridyl)porphyrin, p-aminothiophenol, and benzocaine. Relative standard deviation of SERS signal was found to be less than 20% confirming good spectral reproducibility especially using a 100x objective. Then, we demonstrated sensitive SERS detection of other biologically important molecules adsorbed on our AuFON substrates: thiolated-polyA, protoporphyrin IX, and two alkaloids (nicotine and strychnine). Estimated limits of detection were 8.2x10(-7) and 4.5x10(-7)M (in order of 100ng.ml(-1)) incubation concentration for strychnine and nicotine, respectively. Therefore, we conclude that the 20-nm gold film deposited on polystyrene spheres of 107nm in diameter provided both good spectral reproducibility and sensitivity. Their implementation into the SERS-active system will enable to bring about new quantitative and analytical SERS applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GA13-20110S" target="_blank" >GA13-20110S: Nové substráty a přístupy pro citlivý a reprodukovatelný biomolekulární senzing pomocí spektroskopie povrchem zesíleného Ramanova rozptylu (SERS)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Raman Spectroscopy

ISSN

0377-0486

e-ISSN

—

Svazek periodika

49

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

499-505

Kód UT WoS článku

000426512700012

EID výsledku v databázi Scopus

2-s2.0-85042859063