Towards phosphorus recycling for agriculture by algae: Soil incubation and rhizotron studies using (33)P-labeled microalgal biomass

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F19%3A10403726" target="_blank" >RIV/00216208:11320/19:10403726 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=P_Q_KfT0Jg" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=P_Q_KfT0Jg</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2019.101634" target="_blank" >10.1016/j.algal.2019.101634</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Towards phosphorus recycling for agriculture by algae: Soil incubation and rhizotron studies using (33)P-labeled microalgal biomass

Popis výsledku v původním jazyce

Algae effectively accumulate phosphorus (P) from the environment, qualifying them as a promising novel P fertilizer. We hypothesized that P in algae can be rapidly transformed in soil and mobilized for plant growth. To determine the fate of algal fertilizer in soil and to trace its efficiency for plant uptake, we labeled the algae Chlorella vulgaris with the radioisotope (33)P. To optimize the labeling we studied P-uptake dynamics in detail using a pre-starved culture and additionally monitored polyphosphate (Poly-P) and organic carbon (C) reserve pools by Raman microscopy. Using an optimized labeling procedure, the concentrations and distribution of both algae-derived (33)P and mineral fertilizer (33)P (control) were characterized in incubation and rhizotron experiments. Soil incubation was performed with four major reference groups (Andosol, Alisol, Cambisol, and Vertisol). To assess (33)P plant uptake we grew wheat in rhizotrons on Cambisol. Soil analyses at different incubation times demonstrated sequential (33)P fractionation, while plant uptake of algae-derived (33)P was followed using sequential autoradiographic imaging. We found that the algae increased labile P pools comprising Resin- and NaHCO(3)-extractable P in soils during the first 2 weeks of incubation, similar to the effects of NPK fertilizer. The soils with elevated concentrations of Fe- and Al-oxides (Andosol and Alisol) immediately bound 55 to 80% of the applied fertilizer (33)P into the moderately available NaOH-P fraction, whereas the soils with lower concentrations of Fe/Al-oxides (Cambisol, Vertisol) stored 35-71% of the algal-P in the labile fraction. The rhizotron experiments visually supported the release and plant-uptake of algal (33)P, thus verifying the suitability of algal-fertilizer for plant growth.

Název v anglickém jazyce

Towards phosphorus recycling for agriculture by algae: Soil incubation and rhizotron studies using (33)P-labeled microalgal biomass

Popis výsledku anglicky

Algae effectively accumulate phosphorus (P) from the environment, qualifying them as a promising novel P fertilizer. We hypothesized that P in algae can be rapidly transformed in soil and mobilized for plant growth. To determine the fate of algal fertilizer in soil and to trace its efficiency for plant uptake, we labeled the algae Chlorella vulgaris with the radioisotope (33)P. To optimize the labeling we studied P-uptake dynamics in detail using a pre-starved culture and additionally monitored polyphosphate (Poly-P) and organic carbon (C) reserve pools by Raman microscopy. Using an optimized labeling procedure, the concentrations and distribution of both algae-derived (33)P and mineral fertilizer (33)P (control) were characterized in incubation and rhizotron experiments. Soil incubation was performed with four major reference groups (Andosol, Alisol, Cambisol, and Vertisol). To assess (33)P plant uptake we grew wheat in rhizotrons on Cambisol. Soil analyses at different incubation times demonstrated sequential (33)P fractionation, while plant uptake of algae-derived (33)P was followed using sequential autoradiographic imaging. We found that the algae increased labile P pools comprising Resin- and NaHCO(3)-extractable P in soils during the first 2 weeks of incubation, similar to the effects of NPK fertilizer. The soils with elevated concentrations of Fe- and Al-oxides (Andosol and Alisol) immediately bound 55 to 80% of the applied fertilizer (33)P into the moderately available NaOH-P fraction, whereas the soils with lower concentrations of Fe/Al-oxides (Cambisol, Vertisol) stored 35-71% of the algal-P in the labile fraction. The rhizotron experiments visually supported the release and plant-uptake of algal (33)P, thus verifying the suitability of algal-fertilizer for plant growth.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research

ISSN

2211-9264

e-ISSN

—

Svazek periodika

43

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

101634

Kód UT WoS článku

000489307800033

EID výsledku v databázi Scopus

2-s2.0-85070626295