Drop coating deposition Raman (DCDR) spectroscopy of biologically important molecules

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F23%3A10467642" target="_blank" >RIV/00216208:11320/23:10467642 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=2p_ypaWawP" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=2p_ypaWawP</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jrs.6524" target="_blank" >10.1002/jrs.6524</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Drop coating deposition Raman (DCDR) spectroscopy of biologically important molecules

Popis výsledku v původním jazyce

Drop coating deposition Raman (DCDR) spectroscopy introduces a simple and easily accessible approach to studying biologically important molecules and their mixtures. The method is based on drying a small drop of solution or suspension of studied molecules deposited on a special hydrophobic surface. The drying process efficiently accumulates the molecules in the &apos;coffee-ring&apos; or any small pattern from which the Raman spectrum can be measured. In this way, a significant (several orders of magnitude) improvement of the Raman detection sensitivity compared with Raman measurement from solution can be reached. Therefore, a small sample volume (several microliters), as well as a low initial concentration of studied molecules in deposited droplets, are important advantages of the DCDR method over the normal Raman one. Recently, many relevant DCDR applications on biomolecules and related molecules have been reported. The mini-review covers a brief overview of the DCDR method (principle, short history, suitable hydrophobic surfaces and state-of-the-art). It will be followed by a summary of the studies over the last 15-20 years on different biologically important molecules, including proteins, lipids (in the form of liposomes) and small molecules (e.g., porphyrins, anthrax marker dipicolinic acid and food and environmental contaminants). Finally, the application potential and further perspectives of the DCDR method for biomolecular studies will be discussed.

Název v anglickém jazyce

Drop coating deposition Raman (DCDR) spectroscopy of biologically important molecules

Popis výsledku anglicky

Drop coating deposition Raman (DCDR) spectroscopy introduces a simple and easily accessible approach to studying biologically important molecules and their mixtures. The method is based on drying a small drop of solution or suspension of studied molecules deposited on a special hydrophobic surface. The drying process efficiently accumulates the molecules in the &apos;coffee-ring&apos; or any small pattern from which the Raman spectrum can be measured. In this way, a significant (several orders of magnitude) improvement of the Raman detection sensitivity compared with Raman measurement from solution can be reached. Therefore, a small sample volume (several microliters), as well as a low initial concentration of studied molecules in deposited droplets, are important advantages of the DCDR method over the normal Raman one. Recently, many relevant DCDR applications on biomolecules and related molecules have been reported. The mini-review covers a brief overview of the DCDR method (principle, short history, suitable hydrophobic surfaces and state-of-the-art). It will be followed by a summary of the studies over the last 15-20 years on different biologically important molecules, including proteins, lipids (in the form of liposomes) and small molecules (e.g., porphyrins, anthrax marker dipicolinic acid and food and environmental contaminants). Finally, the application potential and further perspectives of the DCDR method for biomolecular studies will be discussed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Raman Spectroscopy

ISSN

0377-0486

e-ISSN

1097-4555

Svazek periodika

54

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

694-705

Kód UT WoS článku

000970643900001

EID výsledku v databázi Scopus

2-s2.0-85152797575