Immunohistochemical characterisation and PEPSCAN ANALYSIS of monoclonal antibodies against D-type Cyclin-dependent kinase Cdk6

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F00%3A00003043" target="_blank" >RIV/00216224:14110/00:00003043 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immunohistochemical characterisation and PEPSCAN ANALYSIS of monoclonal antibodies against D-type Cyclin-dependent kinase Cdk6

Popis výsledku v původním jazyce

The complexity of regulation of protein Rb interacting with Cdk6, cyclinD and Cdk inhibitors represents a critical checkpoint in cellular signal transduction triggered by mitogenic or antiproliferative stimuli. Such a highly dynamic qurternary complex represents a functional unit that is not only involved in major decisions on the fate of a cell, but is also commonly deregulated in certain types of cancer. Cdk6 protein could be an appropriate marker of proliferation. The aim of the study was to preparea panel of monoclonal antibodies distinguishing different epitopes of Cdk-6 (cyclin- dependent kinase 6) protein. Using hybridoma technology, dot blot, immunoblotting, PEPSCAN ELISA test and immunohistochemical staining, we characterized a panel of monoclonal antibodies against Cdk6 protein. 32 types of hybridoma cells were prepared and subsequently tested for production of monoclonal antibodies. 24 hybridomas were selected and further analyzed for reaction with 21 different target p

Název v anglickém jazyce

Immunohistochemical characterisation and PEPSCAN ANALYSIS of monoclonal antibodies against D-type Cyclin-dependent kinase Cdk6

Popis výsledku anglicky

The complexity of regulation of protein Rb interacting with Cdk6, cyclinD and Cdk inhibitors represents a critical checkpoint in cellular signal transduction triggered by mitogenic or antiproliferative stimuli. Such a highly dynamic qurternary complex represents a functional unit that is not only involved in major decisions on the fate of a cell, but is also commonly deregulated in certain types of cancer. Cdk6 protein could be an appropriate marker of proliferation. The aim of the study was to preparea panel of monoclonal antibodies distinguishing different epitopes of Cdk-6 (cyclin- dependent kinase 6) protein. Using hybridoma technology, dot blot, immunoblotting, PEPSCAN ELISA test and immunohistochemical staining, we characterized a panel of monoclonal antibodies against Cdk6 protein. 32 types of hybridoma cells were prepared and subsequently tested for production of monoclonal antibodies. 24 hybridomas were selected and further analyzed for reaction with 21 different target p

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/VS96097" target="_blank" >VS96097: Molekulární patofyziologie vybraných "civilizačních", multigenně podmíněných chorob</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Cells II

ISBN

80-7232-120-X

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

Kopp nakladatelství

Místo vydání

ČR

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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